转盘反应器固定根霉重复批次发酵生产脂肪酶  

Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizus using rotating biological contactor

作　　者：李丹[1] 张大皓[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院北京市加工过程重点实验室,北京100029

出　　处：《化工学报》2007年第9期2347-2351,共5页CIESC Journal

基　　金：国家自然科学基金项目(50373003;20325622;20576013);国家高技术研究发展计划项目(2002AA217022);北京市自然科学基金项目(2032013);国家重点基础研究发展计划项目(2003CB716002)~~

摘　　要：Production of lipase by Rhizopus arrhizus was studied in a rotating biological contactor(RBC)with polyurethane foam(PUF),as a porous biomass support,attached on both sides of the RBC disks.The effects of aeration rate,rotating rate of discs,volume of broth and the number of discs on the lipase activity were studied in the 6 L RBC,and the optimum fermentation condition was as follows:4 L·min-1 aeration rate,100 r·min-1 rotating rate,3.5 L medium with three discs in 6 L RBC.Repeated batch fermentation by immobilized mycelium was tested and it was found that immobilized cells showed high stability for repeated use.Five repeated batches could be carried out in 6 L RBC and the lipase productivity increased from 3125 U·h-1 in batch fermentation to 9512 U·h-1 in repeated batch fermentation,which was 3 times as high as that in batch fermentation.Production of lipase by Rhizopus arrhizus was studied in a rotating biological contactor （RBC） with polyurethane foam （PUF）, as a porous biomass support, attached on both sides of the RBC disks. The effects of aeration rate, rotating rate of discs, volume of broth and the number of discs on the lipase activity were studied in the 6 L RBC, and the optimum fermentation condition was as follows： 4 L·min^-1 aeration rate, 100 r · min^-1 rotating rate, 3.5 L medium with three discs in 6 L RBC. Repeated batch fermentation by immobilized mycelium was tested and it was found that immobilized cells showed high stability for repeated use. Five repeated batches could be carried out in 6 L RBC and the lipase productivity increased from 3125 U · h^-1 in batch fermentation to 9512 U · h^-1 in repeated batch fermentation, which was 3 times as high as that in batch fermentation.

关 键 词：转盘反应器 重复批次发酵 脂肪酶 根霉 

分 类 号：TQ925[轻工技术与工程—发酵工程]