Leucine-rich repeat C4 protein is Involved in Nervous Tissue Development and Neurite Outgrowth, and Induction of Glioma Cell Differentiation  

作　　者：Minghua WU He HUANG Qiong CHEN Dan LI Zhaoyang ZENG Wei XIONG Yanhong ZHOU Xiaoling LI Ming ZHOU Jianhong LU Shourong SHEN Guiyuan LI 

机构地区：[1]Cancer Research Institute, Central South University, Changsha 410078, China [2]Third Affiliated Hospital ofXiang Ya School of Medicine, Central South University, Changsha 410078, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第10期731-738,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Key Project of Scientific Research Program （Nos. 2006CB910502 and 2006CB910504）, the National Natural Science Foundation of China （Nos. 30770825, 30771135 and 30600224）, the China Postdoctoral Science Foundation （Nos. 20060400265 and 20060390886） and the Hunan Province Natural Sciences Foundations of China （No. 06JJ30014）,Acknowledgement We thank Dr. Naihe JING （Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China） for his gift of the cDNA of neuronal differentiation of embryonic carcinoma P19 cells induced by retinoic acid.

摘　　要：LRRC4, leucine-rich repeat C4 protein, has been identified in human （GenBank accession No. AF196976）, mouse （GenBank accession No. DQ177325）, rat （GenBank accession No. DQ119102） and bovine （GenBank accession No. DQ 164537） with identical domains. In terms of their similarity, the genes encoding LRRC4 in these four mammalian species are orthogs and therefore correspond to the same gene entity. Based on previous research, and using in situ hybridization, we found that LRRC4 had the strongest expression in hippocampal CA1 and CA2, the granule cells of the dentate gyrus region, the mediodoral thalamic nucleus, and cerebella Purkinje cell layers. Using a P19 cell model, we also found that LRRC4 participates in the differentiation of neuron and glia cells. In addition, extracellular proteins containing both an LRR cassette and immunoglobulin domains have been shown to participate in axon guidance. Our data from neurite outgrowth assays indicated that LRRC4 promoted neurite extension of hippocampal neurons, and induced differentiation of glioblastoma U251 cells into astrocyte-like cells, confirmed by morphology observation and glial fibrillary acidic protein expression.LRRC4, leucine-rich repeat C4 protein, has been identified in human （GenBank accession No. AF196976）, mouse （GenBank accession No. DQ177325）, rat （GenBank accession No. DQ119102） and bovine （GenBank accession No. DQ 164537） with identical domains. In terms of their similarity, the genes encoding LRRC4 in these four mammalian species are orthogs and therefore correspond to the same gene entity. Based on previous research, and using in situ hybridization, we found that LRRC4 had the strongest expression in hippocampal CA1 and CA2, the granule cells of the dentate gyrus region, the mediodoral thalamic nucleus, and cerebella Purkinje cell layers. Using a P19 cell model, we also found that LRRC4 participates in the differentiation of neuron and glia cells. In addition, extracellular proteins containing both an LRR cassette and immunoglobulin domains have been shown to participate in axon guidance. Our data from neurite outgrowth assays indicated that LRRC4 promoted neurite extension of hippocampal neurons, and induced differentiation of glioblastoma U251 cells into astrocyte-like cells, confirmed by morphology observation and glial fibrillary acidic protein expression.

关 键 词：LRRC4 DEVELOPMENT neurite outgrowth DIFFERENTIATION GLIOMA 

分 类 号：R739.4[医药卫生—肿瘤]