细菌HGS-3产β-葡萄糖苷酶发酵条件的优化  

Fermentation optimization for the production of β-glucosidase by bacterial strain HGS-3

作　　者：张庆华[1] 李世杰[2] 涂国全[1] 朱红梅[1] 郭晓燕[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]湖北省工业微生物重点实验室,湖北武汉430068

出　　处：《中国酿造》2007年第11期12-16,共5页China Brewing

摘　　要：对细菌HGS-3的产酶条件进行了优化。试验结果表明,栀子甙对细菌HGS-3产酶有着强烈地诱导效果,最佳的产酶条件为碳源(甘蔗渣∶麸皮=3∶1)5%,牛肉膏0.5%,栀子甙0.2%,KH2PO40.4%,MnSO40.04%,pH9.0,装液量20mL,转速200r/min,45℃发酵24h,发酵液中的酶活可达到93.48U/mL,比优化前的酶活16.55U/mL提高了近6倍。The conditions for β-glucosidase production by bacterial strain HGS-3 were optimized. The results showed that the geniposide could intensively induce β-glucosidase production in strain HGS-3. The optimized conditions for β-glucosidase production were： carbon source （bagasse：bran=3：1） 5 %, beef extract 0.5 %, geniposide 0.2 %, KH2PO4 0.4 %, MnSO4 0.04 %, pH 9.0, broth volume 20 ml, rotary velocity 200 r/min, temperature 45% and fermentation time 24h. After optimization, the enzyme activity in broth reached 93.48 U/ml, which was nearly 6 times higher than that before optimization （16.55 U/ml）.

关 键 词：Β-葡萄糖苷酶 发酵条件 优化 

分 类 号：Q814[生物学—生物工程]