重组人锰超氧化物歧化酶高密度发酵培养条件研究  

Optimization of high-cell-density fermentation condition of recombinant human manganese superoxide dismutase

作　　者：陈车生[1] 程贻[2] 吴乾一[2] 袁勤生[2] 吴梧桐[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009 [2]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《中国药科大学学报》2007年第5期472-476,共5页Journal of China Pharmaceutical University

摘　　要：目的:研究发酵罐中采用乳糖诱导高效表达重组人锰超氧化物歧化酶(rhMn-SOD)的条件。方法:通过摇瓶和发酵罐培养研究了培养基、乳糖浓度、诱导时机、表达时间、溶氧条件、补料碳源种类对rhMn-SOD表达的影响。结果:乳糖诱导浓度为0.25%,溶氧浓度为40%～60%,用葡萄糖作为补料碳源进行高密度发酵,菌体密度A600达到28.98,菌体湿重达到60g/L,Mn-SOD活性达到18222.22U/g,比活为1057.5U/mg,实现了rhMn-SOD的高效表达。结论:对培养基配方和发酵条件进行了优化,为rhMn-SOD大规模生产奠定了基础。Aim：To investigate the fermertation condition of lactose-induced recombinant human manganese superoxide dismutase （rhMn-SOD） in the bioreactor. Methods： The influences of culture medias, lactose concentration, inducing times, expression time, dissolved oxygen level and carbon source on rhMn-SOD expression were studyed both in the shake falsks and the bioreactor. Results： In the high-cell-density fermentation, after the bacteria being induced by 0.25% lactose, took glucose as carbon source with the dissolved oxygen controlled between 40% and 60%, the optical-density （A600） of bacteria reached 29.98, and the wet cell weight got 60 g/L. Furthennore, Mn-SOD activity reached 18 222.22 U/g and the specific activity of Mn-SOD was 1 057.5 U/mL. Conclusion： Efficient expression of rhMn-SOD in E. coli was achieved by the optimized fermentation conditions which laid the foundation for its mass production.

关 键 词：重组人锰超氧化物歧化酶 乳糖 高密度发酵 大肠杆菌 

分 类 号：TQ925[轻工技术与工程—发酵工程]