多孔玻璃微珠的制作及其固定α-淀粉酶的研究  

Preparation of Porous Glass Microsphere Carrier and Their Application in the Immobilization of α-Amylase

作　　者：王宝康[1] 

机构地区：[1]湖南农业大学,湖南长沙410128

出　　处：《化工时刊》2007年第11期15-19,共5页Chemical Industry Times

摘　　要：用分相法和填充法分别制作了两种载体多孔玻璃微珠FXBL和TCBL,用共价偶联法分别固定α-淀粉酶,确定了其最佳固定条件和最佳应用条件,并研究了固定化酶的性质。主要结论如下:①最佳固定条件为:温度10℃;pH=6.2;给酶量TCBL 2.6 g/LF、XBL 2.4 g/L;时间12 h;②最佳应用条件为:温度75℃,比自由酶高5℃;pH值TCBL固定化酶为5.2、FXBL固定化酶为5.4,分别比自由酶的最适pH值6.0低0.8和0.6个pH值单位;③固定化酶的主要性质为:在80℃受热1 h,固定化酶的活力下降小于7%,而自由酶活力则下降至63%;FXBL固定化酶和TCBL固定化酶分别使用5次和8次还可以保持60%的活力。Two kinds of porous glass beads, FXBL and TCBL, were prepared by phase separation and stuffing salts. The beads were used as carder for immobilizing α - amylase by convalent binding methods, then the optimum conditions of the immobilization and application were obtained, and the properties of immobilized enzyme were studied. The main conclusion was as followed： （1） The optimal conditions of the immobilization： temperature was 10 ℃, pH was 6.2, the load of enzyme to TCBL and FXBL was 2.6 g/L and 2.4 g/L, time was 12 h. （2） the optimal conditions of the application： temperature was 75℃, it being 5℃ higher as compared to the freea - amylase, pH was 5.2（TCBL as carders） and 5.4（FXBL as carders）, and pH0.8（TCBL as carders） and 0.6（FXBL as carders） unit lower as compared to the free aamylase. （3）The main fundamental properties of immobilized a - amylase： If heated I h at 80 ℃, the activity of immobilized α - amylase would decline less than 7 %, and the activity of free a - amylase would be reduced to 63 %. ff immobilized α - amylase by FXBL and TCBL was reused 5 and 8 times, their activity kept 60 % still.

关 键 词：多孔玻璃微珠 Α-淀粉酶 固定化酶 

分 类 号：TQ920[轻工技术与工程—发酵工程]