胡萝卜抗冻蛋白的新型生物抗冻剂的研制  

New bio-antifreeze from engineered carrot antifreeze protein

作　　者：张党权[1] 谭晓风[1] 彭万喜[2] 刘其梅[2] 马清芝[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004 [2]中南林业科技大学流变力学与材料工程研究所,湖南长沙410004

出　　处：《功能材料》2007年第A05期1984-1986,共3页Journal of Functional Materials

基　　金：湖南省自然科学基金（05JJ40125）：长沙市科技计划重点项目（K069083）

摘　　要：研究了基因工程方法制备的胡萝卜抗冻蛋白在大肠杆菌和水稻悬浮细胞团的低温和超低温冻存实验中提高细胞抗冻的性能．结果表明，在大肠杆菌的冻存实验中，三个处理温度中胡萝卜抗冻蛋白都明显提高了细胞的存活率，与阳性对照基本一致；而在水稻悬浮单细胞的冻存实验中，胡萝卜抗冻蛋白比阳性对照展示了更好的保护细胞的能力，其恢复后的细胞活力和相对细胞活力高，细胞死亡率低，而且在后续复苏培养过程中都能进行有效的子代细胞分裂．实验结果表明，通过基因工程方法大量生产的胡萝卜抗冻蛋白可有效适用于真核细胞的低温保存和超低温保存．The antifreeze effect of carrot antifreeze protein （DcAFP） prepared by gene engineering on E. coli and rice cells treated by cryopreservation was studied. The results showed that gradient concentration DcAFP can distinctly improve survival rate （similar to positive control） of bacteria treated with three temperatures. However, DcAFP displays higher capability of protecting rice cells than positive control during the three temperature cryopreservation, moreover, the cell viability and relative viability derived from DcAFP are higher, but cell death rate is lower than that of positive control, resulting in more effective cell division during the posterior culture. These results indicated that engineered DcAFP can be effectively applicable to the cryopreservation of eukaryotic cells.

关 键 词：抗冻蛋白 抗冻剂 低温保存 细胞活力 基因工程 

分 类 号：Q819[生物学—生物工程]