Effects of Tetrandrine on Apoptosis and Radiosensitivity of Nasopharyngeal Carcinoma Cell Line CNE  

作　　者：Xinchen SUN Ruizhi XU Yuxia DENG Hongyan CHENG Jun M Jiazhong JI Yingfeng ZHOU 

机构地区：[1]Department of Oncology, Zhong Da Hospital Affiliated to Southeast University, Nanjing 210009, China [2]School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第11期869-878,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the Natural Science Foundation of Jiangsu Province （No. BK2005203） and the Developing Program for Medical Technology of Nanjing, China （No. ZKX05030）

摘　　要：Tetrandrine is known to exert antitumor effect, however, little is known about its effect on nasopharyngeal carcinoma cells. In this study, we tested tetrandrine-induced apoptosis and radiosensitivity in nasopharyngeal carcinoma cell line CNE and investigated the possible mechanisms. Using flow cytometry and DNA electrophoresis, we found that tetrandrine could induce cell apoptosis. Further, it was shown that the level of Bcl-2 mRNA decreased and Bax mRNA increased after addition of tetrandrine by using reverse transcription-polymerase chain reaction. X-ray-induced G2 arrest was abrogated by treatment with tetrandrine, as detected by flow cytometry and mitotic index. The accumulation of cyclinB 1 protein and the suppression of Cdc2 tyrosine-15 and Cdc25C serine-216 phosphorylation were detected in irradiated cells treated with tetrandrine using Western blot analysis. Taken together, these results show that tetrandrine can induce apoptosis and abrogate radiation-induced G2 arrest in CNE cells.Tetrandrine is known to exert antitumor effect, however, little is known about its effect on nasopharyngeal carcinoma cells. In this study, we tested tetrandrine-induced apoptosis and radiosensitivity in nasopharyngeal carcinoma cell line CNE and investigated the possible mechanisms. Using flow cytometry and DNA electrophoresis, we found that tetrandrine could induce cell apoptosis. Further, it was shown that the level of Bcl-2 mRNA decreased and Bax mRNA increased after addition of tetrandrine by using reverse transcription-polymerase chain reaction. X-ray-induced G2 arrest was abrogated by treatment with tetrandrine, as detected by flow cytometry and mitotic index. The accumulation of cyclinB 1 protein and the suppression of Cdc2 tyrosine-15 and Cdc25C serine-216 phosphorylation were detected in irradiated cells treated with tetrandrine using Western blot analysis. Taken together, these results show that tetrandrine can induce apoptosis and abrogate radiation-induced G2 arrest in CNE cells.

关 键 词：TETRANDRINE nasopharyngeal carcinoma APOPTOSIS gene regulation RADIOSENSITIVITY 

分 类 号：R739.63[医药卫生—肿瘤]