有机复合物H对纤维素CMC酶活的影响  

Effect of Composite Organic Compounds H on Cellulose Activity of CMC

作　　者：李鸿玉[1] 厉重先[1] 刘雪峥[1] 

机构地区：[1]北京联合大学师范学院,北京100011

出　　处：《生物技术通报》2007年第6期157-161,共5页Biotechnology Bulletin

基　　金：北京市教委科技发展计划面上项目资助

摘　　要：采用3,5-二硝基水杨酸(DNS)为显色剂,CMC为底物,测定纤维素酶的酶活。考察了在不同的酶促反应温度、pH值、底物浓度、反应时间等反应条件下,有机复合物H对纤维素酶CMC酶活(CMCA)的影响。结果表明,有机复合物H对纤维素酶的CMCA活性有明显促进作用,且在不同条件下的促进效果有较大差异。The activity of cellulose be measured with 3,5-dinitrosalicylic acid（DNS）as developer and CMC as the substrate. The effect of composite organic compounds H to CMC cellulose activity be investigated in different temperature,pH,reaction time and the concentration of the substrate material. The results indicate that composite organic compounds H can distinctly increase the activity of CMC and promotive effects have bigger difference in the different conditions.

关 键 词：纤维素酶 有机复合物H 反应条件 CMC 

分 类 号：Q55[生物学—生物化学]