高效液相色谱法测定富含油脂辣椒制品中的辣椒素类物质  

Determination of capsaiciniods in highly oil-containing pepper products by high performance liquid chromatography

作　　者：王穗萍[1] 夏延斌[1] 彭进[2] 郑瑶瑶[1] 熊科[1] 杨峰[1] 陆微微[1] 

机构地区：[1]湖南农业大学食品科技学院,长沙410000 [2]常德市疾病预防控制中心,常德415000

出　　处：《分析试验室》2007年第12期39-43,共5页Chinese Journal of Analysis Laboratory

基　　金：农业部948专项经费(2003T18)项目资助

摘　　要：建立了高效液相色谱法测定高油脂辣椒制品中3种主要辣味成分的方法。样品以70%乙醇提取,采用反相高效液相色谱分离,紫外检测器;根据色谱峰的保留时间定性,以外标峰面积法定量辣椒素（C）、二氢辣椒素（DC）、去甲二氢辣椒素（NDC）,结果以斯氏指数（SHU）计。NDC的色谱行为与C极其类似,NDC可采用C的标准曲线定量。采用色谱柱,ZORBAX SB-C18;流动相,V（甲醇）∶V（水）=70∶30;UV检测波长,230 nm;辣椒素和二氢辣椒素RSD分别为1%,1.5%（n=5）;回收率分别为97.5%、94.5%。辣椒素和二氢辣椒素在0.001-0.1 mg/mL范围内线性良好;LOD 0.1 mg/L,LOQ 0.5 mg/L。供试品溶液稳定,12 h内测定RSD为1%。方法满足辣味水平评价要求。A method was developed for the determination of capsaicinoids in highly oil-containing pepper products by high performance liquid chromatography; so that the pungency level can be measured accurately. The sample was extracted with 70% ethanol. The analysis was performed on a C18 column （250 × 4.6 mm i.d. 5 tan） with 70：30 MeOH-water as the mobile phase at a flow rote of 0.8 mL/min and detected at 230 nm. The column temperature was 30℃. The injection volume was 20 μ/L. The determination of capsaicin （C）, dihydrocapsaicin （DC） and nordihydro- capsaicin （NDC） were processed by peak area and external standard method. The three compounds demonstrated good linear relationship （r 〉 0.999） in the range of 0.001 -0.1 mg/mL. Due to the similar chromatographic behavior, NDC can use the standard curve of C. The LOD was 0.1 mg/L. The LOQ was 0.5 mg/L. The precisions of C and DC were good with the RSDs of 1% and 1.5 % respectively （ n = 5）. The recoveries for C and DC are 97.5 % and 94.5 % respectively.

关 键 词：高效液相色谱 富含油脂 辣椒制品 辣椒素同系物 辣味水平 

分 类 号：O657.7[理学—分析化学]