高效液相色谱法测定发酵醪中的γ-氨基丁酸  

Determination of γ-aminobutyric acid in broth of fermentation by high-performance liquid chromatography

作　　者：杨胜远[1] 陆兆新[1] 焦阳[1] 艾嘉[1] 吕凤霞[1] 别小妹[1] 

机构地区：[1]南京农业大学食品科技学院

出　　处：《广西农业生物科学》2007年第4期331-334,共4页Journal of Guangxi Agricultural and Biological Science

基　　金：国家自然科学基金项目(30671460)

摘　　要：建立了高效液相色谱测定发酵醪中γ-氨基丁酸（γ-aminobutyricacid，GABA）的方法。采用7％（V／V）乙酸水溶液对发酵醪进行预处理，以异硫氰酸苯酯为衍生剂，用反相C18柱为分离柱，柱温27℃，阶段洗脱，在254nm下进行检测。结果表明，发酵醪中的GABA获得了很好的分离，GABA在0～1．5mmol／L内线性相关性好，其线性方程为y=14106．5713x-258．2493（r=0．9993）。最小检测浓度为0．5μmol／L（R5D≤10％），组间样品测定相对误差为3．571％，加样平均回收率达到了99．038％。所建立的方法稳定、灵敏、重现性好，可用于测定发酵醪中的GABA。A method for determination of γ-aminobutyric acid （GABA） in broth of fermentation by high-performance liquid chromatography was established. The broth of the fermentation was firstly pretreated with 7% （V/V） acetic acid, and then was derivatized with phenyl isocyanate （PITC） The ramifications were subsequently separated and assay by HPLC using reversed phase C18 column at 254 nm and 27 ℃. Under the conditions described above, PTC-GABA was well-separated and the calibration curve for PTC-GABA kept linear at 0 -1.5 mmol/L. The regression equation of the calibration curve for PTC-GABA was y： 14106.5713x-258. 2493 （r= 0.9993）. The limit of quantitation, relative error and average recovery of the method were 0.5 μmol/L （RSD≤10%）, 3.571% and 99. 038%, respectively. The results indicated the method described was a sensitive, reproducible and specific assay for the determination of GABA in the broth of fermentation.

关 键 词：高效液相色谱 μ-氨基丁酸 发酵 氨基酸 

分 类 号：TQ920[轻工技术与工程—发酵工程]