考马斯亮蓝G-250法快速测定牛乳中的蛋白质  

Quick determination of protein in fresh milk with Coomassie Brilliant Blue G-250

作　　者：南亚[1] 李宏高[1] 

机构地区：[1]陕西科技大学职业技术学院,陕西西安710016

出　　处：《饮料工业》2007年第12期41-42,44,共3页Beverage Industry

摘　　要：依据考马斯亮蓝G-250与蛋白质结合呈现蓝色,在595nm波长下的吸光度与蛋白质含量呈正比,制作出标准曲线。研究了最佳实验条件,成功地建立一个牛乳中蛋白质快速测定的方法,具有简单、快捷、易行等特点。结果表明该方法精密度高,RSD为1.70%,结果准确,相对误差较小,回收率98.2%￣100.3%。A calibration curve can be drawn in accordance with the theory that the mixture of Coomassie Brilliant Blue G-250 with protein shows blue colour, and that the content of protein goes in direct ratio with the absorbance of Coomassie Brilliant Blue G-250 with the wavelength of 595nm. In the thesis, the determination of the optimum experimental conditions is focused on so as to find out a quick, simple and easy method of determining the content of protein in fresh milk. The results show that the method has a high degree of precision （RSD 1.7%） and little relative error with a recovery coefficient of 98.2%-100.3%.

关 键 词：考马斯亮蓝 牛乳 蛋白质 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]