固定化诺卡氏菌批次连续转化生产L(+)酒石酸  

Repeated-Batch Production of L(+) Tartaric Acid by Immobilized Nocardia sp.Cells

作　　者：黄磊[1] 龙琼英[1] 李志敏[1] 叶勤[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2007年第6期794-797,860,共5页Journal of East China University of Science and Technology

基　　金：上海市科委项目(03DZ19207)

摘　　要：采用卡拉胶包埋具有环氧琥珀酸水解酶活性的诺卡氏菌用于生产L(+)酒石酸。为了提高细胞固定化的效率,采用自制的固定化装置,将固定化细胞制成细条状,可以加快固定化细胞的制备,获得具有高活性和强度的固定化细胞颗粒,固定化细胞的最佳菌体浓度为100 g(湿细胞)/L。将固定化细胞用于填充床反应器进行反复批式反应,可以反复利用48次以上,将0.85 mol/L的环氧琥珀酸溶液转化为L(+)酒石酸,酒石酸的得率不低于96%。k-Carrageenan was used to immobilize Nocardia sp. ceils with epoxysuccinate hydrolase activity for the production of L（＋）tartaric acid. A specially designed equipment was used to immobilize Nocardia cells in the form of strips. Preparation of immobilized cells was quicker and the obtained earageenan strips were more active and rigid. The strips of immobilized Nocardia cells retained the highest epoxysuccinate hydrolase activity at a wet cell content of 10g （wet cell weight）/L. Repeated batch production of L（＋） tartaric acid was carried out with the strips of immobilized cells in a batch plug flow reactor （BPFR） at an initial cis-epoxysuccinate concentration of 0.85 mol/L. L（＋） tartaric acid was formed at a yield no less than 0.96 in 48 consecutive batch reactions.

关 键 词：L(+)酒石酸 诺卡氏菌 环氧琥珀酸水解酶 固定化细胞 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]