基质固相分散-高效液相色谱法测定兔肉中四环素类药物多残留  

MSPD Clean-up and HPLC Determination of Tetracyclines in Rabbit Muscle

作　　者：宋欢 林勤保[2] 连庚寅 韩兵兵[2] 杜利君 

机构地区：[1]山西出入境柃验检疫局技术中心,山西太原030024 [2]山西大学现代化学研究所,山西太原030006

出　　处：《食品科学》2008年第1期250-253,共4页Food Science

基　　金：山西省青年科技基金项目(20041030)

摘　　要：建立了兔肉中四环素类药物的MSPD-HPLC-UV分析法。本实验通过样品和C18填料研磨混合、装柱、淋洗、富集、反相HPLC测定,建立了同时检测兔肉中土霉素、四环素、金霉素、强力霉素4种四环素类药物多残留的基质固相分散-液相色谱分析方法。色谱测定采用C18柱分离,以0.01mol/L草酸-乙腈为流动相,梯度洗脱,流速为1.0ml/min,用紫外检测器于365nm波长下检测。该方法的检出限为0.01μg/g,定量限为0.05μg/g,加标回收率为69.8%～103.1%,相对标准偏差在1.2%～21.8%之间。结果表明,该方法具有灵敏、准确、简便、检测限低等优点,适用于畜产品中上述4种四环素类抗生素残留的同时检测。The MSPD-HPLC-UV method for simultaneous isolation and detection of four tetracyclines（oxytetracycline, tetracycline, chlortetracycline and doxycycline）in rabbit muscle was developed. Blank samples and tetracyclines fortified rabbit muscle samples were ground with C18 filler in half solid state to pack the column, drip with washing, and determine by HPLC. In the method, the mobile phase is 0.01 mol/L oxalic acid and acetonitrile （85：15） with gradient elution, the flow rate 1.0 ml/min, with the detection wavelength 365 nm, and the injection volume 50 μl. The limit of quantitation is 0.05 μg/g. Overall recoveries are between 68.9% and 103.1%. The RSD ranges from 1.2% to 21.8%. The results showed that the method is sensitive, accurate, convenient with low examination limits, which can be used to detect simultaneously the 4 tetracyclines residues in animal products.

关 键 词：兔肉 四环素类药物 基质固相分散(MSPD) 高效液相色谱(HPLC) 

分 类 号：TS207[轻工技术与工程—食品科学]