4种富硒植物预防MNNG诱发大鼠胃癌效果研究 二、不同源硒预防大鼠胃癌的安全性对比研究  

Preventive effects of 4 Se-enriched plants on rat stomach cancer induced by MNNG 2.Safety of different selenium resources for prevention of stomach cancer

作　　者：杨文婕[1] 陈竞[1] 李卫东[1] 陈晓滨[1] 曲宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2008年第1期90-92,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No30170800)

摘　　要：目的对比研究长期摄入高剂量不同源硒的安全性。方法以亚硒酸钠为对照硒材料,采用N-甲基-N′-硝基-N-亚硝基胍(MNNG)诱发大鼠胃癌模型,连续灌以4种不同富硒植物(高剂量硒)17周,测定肝脏谷胱甘肽硫转移酶(GST)、血清谷草转氨酶(AST)和血清谷丙转氨酶(ALT)活性,并观察肝脏和肾脏的病理变化。结果各组大鼠肝GST活性均无显著性差异;75μg/kg bw(以Se计,下同)亚硒酸钠低剂量组大鼠血清AST和ALT活性不仅显著高于空白对照和MNNG组,而且显著高于150μg/kg bw和300μg/kg bw植物硒处理组(P<0.05)。病理分析发现75μg/kg bw亚硒酸钠低剂量组胆管周围有棕黄色颗粒,窦内枯否氏细胞轻度肥大、增生;其余各组未发现有意义的病变。结论亚硒酸钠毒性至少是实验用其它富硒植物的4倍。Objective To guide the application of selenium （Se） in the prevention of human chronic diseases, the safety of different Se resources （selenite, Se-enriched garlic, Se-enriched broccoli, Se-enriched green kale and Seenriched red kale） was investigated. Methods Ninety rats were fed the basal diet for a week, divided equally into the control, N-methyl- N＇-nitro-N-nitrosoguanidine （MNNG） and seven Se treatment groups. Rats were daily given 15mg/kg bw of MNNG for ten days except those in the control group, and rats in seven Se supplementation groups were daily given 75μg/kg bw or 150μg/kg bw sodium selenite solution, 150 or 300μg/kg bw Se as of Se-enriched plant suspension by garage for 17 weeks. Rats were sacrificed at the end of 18th week, the liver glutathione S-transferase（GST）, serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） activities were detected. Rats were given with the basal diet and water freely during the experiment. The data was statistically analyzed by SPSS 12.0 software. Results All rats in Se 75μg/kg bw of Se as sodium selenite group died within 6 weeks, the body weights of rats in 75μg/kg bw of Se as sodium selenite group were significantly lower than those of rats in other groups, the serum AST and ALT activities of rats in 75μg/kg bw of Se as sodium selenite group were significantly higher than those of rats in other groups, and only the Se supplementation of 75μg,/kg bw of Se as selenite led to rat liver pathological change. Conclusion Selenite is at least 4 times as toxic as Se-enriched higher plants selected in this experiment.

关 键 词：富硒植物 硒安全性 MNNG大鼠胃癌模型 微量元素 硒 

分 类 号：R735.2[医药卫生—肿瘤] Q581[医药卫生—临床医学]