抗菌肽Lactoferricin基因的PCR合成及克隆载体T-Lfn的构建  

PCR synthesization and construction of cloning vector T-Lfn of antimicrobial peptide Lactoferricin gene

作　　者：李铁晶[1] 黄占权[1] 许岩[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第1期107-111,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省青年基金(QC06C015)

摘　　要：Lactoferricin是一种天然抗菌肽。试验进行了Lactoferricin基因的PCR合成,将其引入质粒,转化E.coliJM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因克隆载体T-Lfn。Lactoferricin is a kind of natural antibacterial peptide. In this study, the Lactoferricin gene was synthesized with PCR, linked with the plasmid of pMD18-T Vector, and transformed into E.coli JM-109. The identifications of PCR, enzymical degestion and sequencing were carried out, and the cloning vector of T-Lfn was successfully constructed.

关 键 词：抗菌肽 乳铁素 载体构建 

分 类 号：Q781[生物学—分子生物学] Q785