菠萝半纤维素多糖的提取纯化及免疫活性研究  

Extraction, Purification and Immunobiological Activity of Pineapple Hemicellulose Polysaccharide

作　　者：严浪[1] 田海军[1] 张树明[1] 张凡华[1] 李全宏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2008年第2期35-38,共4页Food Science

摘　　要：目的:从菠萝皮渣中的分离得到半纤维素多糖,初步研究其结构及免疫学活性。方法:采用EDAE-Sepharose Fast Flow离子交换柱色谱和Sepharose CL-6B凝胶过滤柱色谱分离纯化,得到菠萝半纤维素多糖PAP4-II。高效液相凝胶渗透色谱检测其均一性及分子量,高效液相色谱、紫外光谱、红外光谱对多糖结构进行初步分析,体外T、B淋巴细胞增殖实验研究多糖的生物学活性。结果:菠萝半纤维素多糖PAP4-II的分子量为1690kD,由木糖和阿拉伯糖两种单糖组成,含量分别为54.02mg/g和39.32mg/g,不含游离或结合的核酸、蛋白类物质,含有糖醛酸。体外细胞实验显示,PAP4-II对T、B淋巴细胞增殖表现出抑制作用。结论:PAP4-II是首次从菠萝皮渣中分离得到的酸性杂多糖。A hemicellulose polysaccharide coded as PAP4-Ⅱ was isolated from pineapple pomace by alkaline-water extraction and purified by ion change chromatography and size exclusion chromatography, and its immunobiological activity was assayed. PAP4-Ⅱ is an acidic compound containing 4.75% protein and 55.13% polysacchadde. Its structural characteristics were investigated by HPLC, UV and IR. Based on the data obtained, PAP4-Ⅱ consists of xylose and arabinose, and their contents are 54.02 mg/g and 39.32 mg/g respectively. It contains uronic acid with a molecular weight of 1690 kDa. The immunobiological activity of PAP4-Ⅱ was studied by T and B lymph cells experiment. Results showed that it has inhibiting effects on the increase of T and B lymph cells.

关 键 词：菠萝 多糖 纯化 结构分析 免疫活性 

分 类 号：Q539.5[生物学—生物化学] R967[医药卫生—药理学]