血小板源生长因子对大鼠血管平滑肌细胞表达血管细胞粘附分子1的影响  

Effects of Platelet-derived Growth Factor on Expression of Vascular Cell Adhesion Molecular 1 in Vascular Smooth Muscle Cell

作　　者：于悦卿[1] 史英钦[1] 郭文潮[1] 余小平[1] 郝玉宾[1] 

机构地区：[1]河北省人民医院遗传研究室,河北省石家庄市050051

出　　处：《中国动脉硬化杂志》2007年第11期851-853,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的本实验研究血小板源生长因子对血管平滑肌细胞表达血管细胞粘附分子1的影响以及对血管平滑肌细胞与单核细胞粘附的作用。方法利用基因芯片和RT-PCR技术检测鼠主动脉血管平滑肌细胞经血小板源生长因子处理0min、20min、6h后血管平滑肌细胞表达血管细胞粘附分子1的mRNA表达变化;细胞粘附实验观察血管平滑肌细胞经血小板源生长因子分别处理0min、20min、6h后与单核细胞粘附情况。结果血小板源生长因子对鼠血管平滑肌细胞表达血管细胞粘附分子1的表达有显著的诱导作用,其诱导作用在20min即已出现(P<0.05),6h时明显增强(P<0.01)。细胞粘附实验显示随着血小板源生长因子的作用时间的延长,血管平滑肌细胞与单核细胞的粘附率增高,血小板源生长因子处理20min和6h后粘附率是对照组的1.92倍和3.04倍(P<0.05)。结论血小板源生长因子明显诱导血管平滑肌细胞中血管平滑肌细胞表达血管细胞粘附分子1的表达,促进血管平滑肌细胞与单核细胞的粘附,从而参与了损伤早期,白细胞向内膜下迁移的炎症反应。Aim To investigate the effects of platelet derived growth factor（PDGF） on the expression of vascular cell adhesion molecular-1（VCAM-1） and the adhesion of monocyte-VSMC.Methods GEArray Gene Array and RT-PCR technique were used to analyse the mRNA expression of VCAM-1 in VSMC after incubated with PDGF for 0 min、20 min、6 h.Meanwhile,the adhesion of monocyte-VSMC was detected by adhesion assay.Results The mRNA expression of VCAM-1 was significantly induced by PDGF,as compared with control group. PDGF-induced VCAM-1 accumulation was appeared at 20 min, and significantly increased at 6 h. PDGF also enhanced the adhesion rate of monocyte and VSMC. Conclusions The results demonstrated that PDGF significantly induced the expression of VCAM-1 in VSMC and enhanced monocyte bind to VSMC. This suggested that PEGF played an important role in early injured lesion and contribution to monocytes in circulation adheresion and migration across the endothelium.

关 键 词：病理学与病理生理学 炎症反应 血小板源生长因子 血管平滑肌细胞 血管细胞粘附分子1 单核细胞 

分 类 号：R363[医药卫生—病理学]