噬菌体展示技术在食品真菌毒素检测领域的应用  

Phage Display Technique and It,s Application in Food safety

作　　者：张园园[1] 裴世春[1] 郑东和[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,食品安全专业黑龙江大庆163319

出　　处：《中国农学通报》2008年第3期87-90,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"DON 和 AFB1 的单链抗体基因重组及其活性研究"(No.30771799);黑龙江省教育厅项目"DON 和 AFB1 真菌毒素检测双功能重组单链抗体的制备研究"(No.1151hz025)

摘　　要：噬菌体展示技术(Phage display technology)是20世纪80年代逐步建立并发展起来的一项分子生物学新技术。它以噬菌体或噬粒为载体,使外源肽或蛋白基因与噬菌体表面特定蛋白基因在其表面进行融合表达,进而通过亲和富集法筛选表达有特异肽或蛋白质的噬菌体。利用噬菌体表面展示技术可以构建抗体库、随机肽库、蛋白质突变体库和cDNA文库,从而广泛用于单链抗体的筛选、先导化合物的筛选、疫苗的研制等,尤其是在单链抗体、模拟抗原表位的筛选及建立食品安全快速检测体系方面,具有广阔的应用前景。本文跟踪了目前噬菌体展示技术在真菌毒素检测领域的最新研究进展和发展前景。Phage display technology is a new molecular biology technology, which is gradually established and developed since 1980s. Its vector is phage or plasmid, exogenous peptide or protein gene and definite protein on super of phage fuse and express, screen phage which express idio-peptid or protein though affinity enriching. To use phage display construction antibody library, trabant peptide library, protein mutant library and cDNA library, thus generally using this technology to screen single chain antibody, leader compound, develop vaccine et al. The technology has extensive application perspective, especially in screening single chain antibody, mimic epitope and food safety detection system approach. This paper tracked the present phage display technology newest research progress in mycotoxins and the development prospects.

关 键 词：噬菌体展示技术 食品安全 单链抗体 模拟表位 

分 类 号：R446.61[医药卫生—诊断学]