Serratia sp. PS-2产几丁质酶发酵条件研究  

Optimization of fermentation conditions for chitinase production by Serratia sp.PS-2

作　　者：潘志强[1] 黄惠琴[1] 单群[2] 鲍时翔[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所 [2]徐州师范大学生命科学院,徐州221116

出　　处：《工业微生物》2008年第2期47-51,共5页Industrial Microbiology

摘　　要：采用平板透明圈法,从海洋红树林根泥筛选得到一株产几丁质酶较强沙雷氏菌株Ser-ratiasp.PS-2。为实现工业化生产几丁质酶,考察了以几丁质为诱导剂,碳源、氮源、NaCl浓度、培养温度、时间、培养液酸碱度、通气量和搅拌速度等对产酶的影响,得到了优化的发酵条件。5L发酵罐实验表明,该菌株可在发酵72h内达到产酶高峰,最高酶活力可达到0.68 U/mL。Serratia sp. （PS-2） with high chitinase activity was isolated from oceanic environment. Experiments were designed to optimize the fermentation parameters, including the component concentrations of carbon source, nitrogen source, NaCl, and the fermentation conditions of temperature, time, pH, ventilation and stirring speed. Finally, fedbatch culture process of PS-2 was carried out to produce chitinase in 5L bioengineering autocontrol fermentor. The highest activity of enzyme could reach 0.68 U/mL.

关 键 词：沙雷氏菌 几丁质酶 发酵 相对酶活力 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]