低能氮离子注入香菇生物学效应的研究  

Study of biological effects on Lentinula edodes by N^+ ion implantation of low energy

作　　者：鹿桂花[1] 周海燕[1] 陈恒雷[1] 张军[1] 吕杰[1] 

机构地区：[1]新疆大学离子束生物工程中心,乌鲁木齐830008

出　　处：《辐射研究与辐射工艺学报》2008年第2期85-88,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家发改委高技术产业化示范工程项目([2004]2077)资助

摘　　要：本文用能量为15keV,而注量不同的氮离子注入对武香一号香菇进行诱变,分析了不同注量下菌丝长速,菌丝生物量以及多糖含量的变化,对该低能离子与微生物间的作用规律进行了初步探索,为进一步筛选出多糖高产菌株提供前提。实验结果表明菌丝长速,菌丝生物量及多糖含量随不同注量的变化情况基本遵循"马鞍型曲线"。注量小时菌丝日均长速较快,随着注量的增大,菌丝日均长速呈下降趋势,但在1.5×1016cm-2附近,菌丝长速有短暂回升。在注量较小时,随着注量的增加,菌丝生物量及多糖含量先升高后降低,注量为4×1015cm-2时,两者均达到最大值,而后会出现小的波动,注量增加到1.5×1016cm-2时,菌丝生物量及多糖含量又会达到一个较大的峰值。通过发酵动力学分析,研究了香菇液体菌种培养过程中菌丝球数量及形态、摇瓶总重、pH值、多糖含量等指标的变化情况,绘制该菌发酵产糖的过程曲线,这将为诱变前后液体发酵过程的控制提供参数。Lentinula edodes were implanted with various nitrogen ions implantation dosage but the same energy in this study. The variations of growth rate of mycelium, mycelial biomass and the contents of lentinan（LNT） under various nitrogen ions implantation dosage were investigated. Three curves of implantation all take a ＂saddle shape＂ with increasing N^＋ ion implanted doses. The growth rate of mycelium is high under low dosage of N^＋ implantation, but with the increment of dosage, the growth rate shows downtrend then began rising when the dosage reaching 1.5×10^16 cm^-2. The mycelia biomass and the contents of LNT rise firstly and then fall with the increment of dosage, and they both reach maximum at the dosage of 4×10^15 cm^-2. Some fluctuation exists in the process, so they both reach another peak at the dosage of 1.5×10^16 cm^-2. During the liquid culture of Lentinula edodes, the variation of mycelial biomass, gross weight of shake-flask, pH and the contents of lentinan（LNT） has been studied. The consistency between the variation of mycelia biomass and gross weight of flask during the liquid culture has also been confirmed. This result can be applied to the study on fermentation before and mutation. Thus a conclusion may be drawn that it is an effective way of mutation breeding by N^＋ ion implantation.

关 键 词：香菇 离子注入 诱变 发酵 多糖 

分 类 号：S335[农业科学—作物遗传育种]