粮食中麦角甾醇含量正相液相色谱法检测  

DETERMINATION OF ERGOSTEROL IN GRAIN——NORMAL PHASE HPLC METHOD

作　　者：何学超[1] 郭道林[1] 兰盛斌[1] 张华昌[1] 黎万武[1] 丁建武[1] 冯永建[1] 

机构地区：[1]国家粮食局成都粮食储藏科学研究所,成都市花牌坊街95号610031

出　　处：《粮食储藏》2008年第2期43-46,共4页Grain Storage

基　　金：国家科技部基础平台项目;粮食中麦角甾醇含量检测技术体系建立与研究(项目编号:2003DIB4J134)

摘　　要：介绍了一种可准确、快速测定粮食中麦角甾醇含量的液相色谱方法。样品皂化后,用正己烷萃取麦角甾醇,不需要进一步纯化,直接上液相色谱分析。分析柱为Spherisorb硅胶柱,检测波长282nm。方法的检出限为0.1mg/kg,方法的回收率在95.1%～100.0%。分析的准确度评价采用微量制备技术和反相液相色谱分离技术,证明了该方法分析的结果准确可靠。A sensitive,rapid ,reproducible and reliable liquid chromatographic method is described for determination of ergosterol in grain. The sample is saponified directly and the saponified mixture is extracted with n -hexane. Ergosterol is determined without further purification or clean up steps by using a liquid chromtagraph with a 250 × 4.0 mm column packed with spherisorb Si, 5μm, and a high pressure column prefilter. The ultraviolet detector is set at 282 nm. The limit of detection was 0.1 mL/m^3;recovery ranged 95.1% 100.0 %. The data analysed by this method is accurate, demonstrated by mini-preparative-HPLC method and reverse phase HPLC method.

关 键 词：粮食 麦角甾醇 正相液相色谱法 

分 类 号：TS207[轻工技术与工程—食品科学]