松茸、蛹虫草混菌共酵菌丝体多糖提取工艺的优化研究  

STUDY ON THE OPTIMIZATION OF THE WATER EXTRACTION TECHNIQUE OF TRICHOLOMA MATSUTAKE AND CORDYCEPS MILITARIS CO-CULTIVATING MYCELIUM POLYSACCHARIDES

作　　者：吴漾[1] 李婷婷[1] 李永乐[1] 姚远超[1] 滕利荣[1] 逯家辉[1] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《食品研究与开发》2008年第5期24-28,共5页Food Research and Development

基　　金：吉林大学国家级生物实验教学示范中心设计创新实验基金一类资助项目(ZXCX2006001)

摘　　要：在单因素实验的基础上,采用响应面分析法研究了提取温度和时间、不同水料比对松茸、蛹虫草混菌共酵菌丝体多糖提取率的影响,建立了具有良好预测性能的常规水浸提工艺条件的数学模型,通过响应面分析法确定了混菌共酵菌丝体多糖的最优提取工艺条件。实验结果表明,水料比对混菌共酵菌丝体多糖提取率的影响显著,最优提取工艺条件为:提取温度64℃,提取时间3h,水料比38:1(mL:g)。经验证实验验证,在该最佳工艺条件下混菌共酵菌丝体粗多糖的提取率可达18.93%,与理论计算值19.07%基本一致,该模型可较好地预测松茸、蛹虫草混菌共酵菌丝体多糖的提取率。Single factor tests and Response Surface Methodology （RSM） based on a three-level three-factor Box-Behnken design were applied to optimize the water extraction conditions of Tricholoma matsutake and Cordyceps Militaris co-cultivating mycelium polysaccharides. The optimum extraction conditions confirmed by the regression analysis were as follows：extracting temperature 64 ℃, extracting time 3 h and the ratio of water to sample 38：1. In this study, the optimized extraction conditions produced theoretically extraction rate of 19.07 % and in practice 18.93 %. The good agreement between the predicted and experimental results verified the validity of the model and existence of an optimal point.

关 键 词：松茸 蛹虫草 共酵菌丝体多糖 水浸提 响应面法 

分 类 号：R284[医药卫生—中药学]