栓菌属漆酶催化儿茶素和绿原酸生物转化的动力学研究  

STUDY ON ENZYMATIC KINETICS OF TRAMATES VERSICOLOR LACCASE CATALYSED CATECHIN AND CHLOROGENIC ACID BIO-TRANSFORMATION

作　　者：左莹[1] 马惠玲[1] 罗耀红[1] 苏钰琪[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《食品研究与开发》2008年第5期39-43,共5页Food Research and Development

摘　　要：为了掌握漆酶对不同种类苹果酚类化合物生物催化的特异性,以白腐菌漆酶为试材,儿茶素和绿原酸为模式底物进行了酶促反应动力学参数的测定。结果表明,漆酶催化儿茶素、绿原酸的转化产物最大吸收波长分别为442nm、379nm;反应最适温度均为55℃;最适pH对儿茶素为pH6.0,pH6.5两个值,对绿原酸为pH5.5,pH6.0两个值;儿茶素浓度0.5mol/L时,最适酶浓度为0.05U/mL,2种底物的漆酶催化反应均符合米氏方程规律。在25℃温度条件下测得儿茶素和绿原酸两种底物的Km值分别为0.1489,0.3390mol/L;Vmax分别为212.77,28.01δA/(U·min)。这为苹果加工中漆酶催化混合酚类化合物转化工艺的确定提供了依据。Trametes versicolor laccase was employed to determine enzymatic kinetics parameters for model substrates, catechin and chlorogenic acid, in order to learn the specific of bio-transformation of different apple phenolic compounds catalyzed by Laccase.. The results showed that the maximum absorption wavelength of laccase-catalyzed end-product for catechin and chlorogenic acid were 442 nm,379 nm, respectively; optimum reaction temperatures were both 55 ℃ ;optimum pH were pH6.0 and pH6.5 for catechin,pH5.5 and pH6.0 for chlorogenic acid; enzyme concentration of 0.05 U/mL was the most efficient for 0.5 mol/L catechin. And laccasecatalyzed bio-transformation for both substrates fitted the Michaelis-Menten equation. Under the reaction temperature of 25 ℃ Km values for catechin and chlorogenic acid were 0.148 9,0.339 0 mol/L, respectively; Vmax values were 212.77, 28.01 8A/（U ·min）, respectively. All the parameters obtained will supply a basis for the bio-transformation technoledge of mixed phenols necessary during apple proceccing.

关 键 词：漆酶 生物催化 儿茶素 绿原酸 酶动力学 

分 类 号：TS201.3[轻工技术与工程—食品科学]