等梯度高效液相色谱法检测食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的方法研究  

DETERMINATION OF SUDAN Ⅰ, Ⅱ, Ⅲ, Ⅳ IN FOODS BY CONSTANT GRADIENT REVERSED-PHASE HPLC

作　　者：刘然[1] 万守朋 

机构地区：[1]天津市工业微生物研究所,天津300462 [2]天津市利民调料有限公司,天津300308

出　　处：《食品研究与开发》2008年第5期107-110,共4页Food Research and Development

摘　　要：建立了等梯度反相高效液相色谱法检测食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的方法。样品用乙腈提取,C18作为固定相,乙腈作为流动相,检测器采用紫外可见光检测器。结果:该方法线性范围为0.15μg/mL～7.5μg/mL,相关系数均为0.999以上(n=7),最低检出限为0.01μg/mL,回收率达到98%～102%。结论:该方法操作简便、快捷、灵敏度高,适用于一般实验室进行食品中苏丹红的检测。A method for determination of Sudan Ⅰ , Ⅱ, Ⅲ, Ⅳ in food by constant gradient reversed-phase high performance liquid chromatography was established in this paper. The sample was treated with acetonitrile, a Hydrosphere ODS-C18 column with acetonitrile as mobile phase and UV-visible light detector.The calibration curve was linear over a range from 0.15 μg/mL to 7.5 μg/mL with a coefficient over 0.999 （n=7）. The lowest detected limit was 0.01 μg/mk The recoveries were from 98 % to 102 %. This method had these advantages such as celerity, high effect and high sensitivity, which coule be used in lab for the determination of Sudan in food.

关 键 词：等梯度 高效液相色谱法 食品 苏丹红 

分 类 号：TS207[轻工技术与工程—食品科学]