额尔齐斯河野生丁鱥肠道产蛋白酶菌株的初步鉴定及产酶条件的研究  

Primary identification of produce-proteinase strains and study on proteinase secrete condition of wild Tinca tinca L in Ereqsi River,Xinjiang

作　　者：冯新忠[1] 王咏星[1] 范镇明 苏俊[1] 苟萍[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]新疆兵团水产技术推广站,新疆乌鲁木齐830002

出　　处：《食品工业科技》2008年第4期104-106,共3页Science and Technology of Food Industry

基　　金：新疆生产建设兵团科技攻关计划项目(2006GG18)

摘　　要：采用酪素培养基和脱脂乳培养基筛选额尔齐斯河野生丁鱥肠道内产蛋白酶菌株,共获得40株蛋白酶产生菌,其中8株蛋白水解圈较大,菌株编号为P1～P8。选取水解圈直径最大的P5为出发菌株,进行生理生化鉴定,同时摇瓶发酵探讨最佳产酶条件。结果:生理生化反应将P5初步鉴定为芽孢杆菌;蛋白酶最适产酶条件为:40℃、初始pH7.0、接种量3%、15mL/250mL的装液量、180r/min、培养时间35h,最大产酶量168.3U/L;添加微量Mg2+、Mn2+、Ca2+有稳定酶活的作用。Screening produce-proteinase strains from wild Tinca tinca L of Ereqsi River, Xinjiang by casin mediaand skimmed milk media obtained 40 strains, 8 strain produced some bigger hydrolyse zone and strains designated as P1-P8.Chosen P5 of bigest hydrolyse zone as start strain and P5 was identified with physiological cultures. It was shown that P5 was primarily identified as Bacillus sp. Under optimum proteinase secrete condition, 40℃, primary pH7.0, inoculation dose 3%, 15mL/150mL volume, 180r/min, 35h and maximum yields was 168.3U/L. with trace Mg^2＋ ,Mn^2＋ and Ca^2＋ could promote stable proteinase activity.

关 键 词：丁鱥 蛋白酶 产酶条件 初步鉴定 额尔齐斯河 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]