猪血红蛋白的酶解及氨基酸含量的研究  

Studies on the enzymatic hydrolysis of porcine hemoglobin and the content of amino acids in the hydrolysate

作　　者：董清平[1] 方俊[1] 蒋红梅[1] 卢向阳[1] 

机构地区：[1]湖南农业大学生科院

出　　处：《现代生物医学进展》2008年第7期1259-1261,共3页Progress in Modern Biomedicine

基　　金：中国博士后基金项目(20070410984);湖南省教育厅重点项目(07A029);湖南省科技重大专项(2007FJ1003);校创新团队项目(04TD01)

摘　　要：猪血是一种营养价值高而价格低廉的蛋白质资源。猪血中含有丰富的蛋白质,其中血红蛋白占主要部分。因此,对血红蛋白及其水解产物的研究受到了国内外众多学者的关注。为选出适合于水解猪血红蛋白的蛋白酶,笔者采用碱性蛋白酶、木瓜蛋白酶、中性蛋白酶对猪血进行单酶和复合酶水解试验,确定碱性蛋白酶为水解猪血的适用酶。通过质谱仪测定,碱性蛋白酶酶解产物分子量集中在800～2400道尔顿,而800～1260道尔顿区间占主要部分;经全自动氨基酸分析仪测出其产物含有17种氨基酸,其中含8种必需氨基酸,必需氨基酸占总氨基酸量的50.93%,说明碱性蛋白酶酶解产物是制作食品和饲料添加剂的理想资源。Porcine blood is a cheap protein resource and its nutrition value is very high. There is plenty of protein in porcine blood and the primary proportion is hemoglobin. Therefore, researches on hemoglobin and its hydrolysate have been paid more attention in domestic and abroad. In order to find the best protease for enzymatic hydrolysis of porcine hemoglobin, a hydrolysis was conducted by the single and combination of alcalase, papain and neutral protease. The results showed that alcalase was the optimal protease for enzymatic hydrolysis of porcine hemoglobin. The hydrolysate of alcalase measured by mass spectrograph in range of 800-2400 kDa MW was obtained, and the hydrolysate in range of 800-1260 kDa MW account for a great many. The hydrolysate of alcalase tested by automatic amino acid analyzing showed that it contains 17 kinds of amino acids and in which 8 kinds of essential amino acids amount to 50.93% of total amino acids. This result showed that the hydrolysate ofalcalase was perfect resource of additive of making foodstuff and feedstuff.

关 键 词：猪血 血红蛋白 酶解 

分 类 号：Q93-331[生物学—微生物学]