两种双酶切大豆分离蛋白技术路线的比较  

Comparison of Two Technologies for Soybean Protein Isolate Two-Enzymatic Hydrolysis

作　　者：宋春丽[1] 迟玉杰[1] 

机构地区：[1]大豆生物学教育部重点实验室,东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2008年第5期65-68,共4页Food Science

基　　金：国家“863”计划项目(2006AA10Z332)

摘　　要：本研究以碱性丝氨酸蛋白酶和蛋白酶Ⅱ作为水解酶对大豆分离蛋白进行两步定向酶切,以制备具有改善肝昏迷生物活性的高F值寡肽。本研究设定两种定向酶切的技术路线,即在酶解过程中维持碱性丝氨酸蛋白酶最适pH的时间分别设定为3h(技术路线Ⅰ)和30min(技术路线Ⅱ),而蛋白酶Ⅱ分别在酸性及中性条件下继续酶解大豆分离蛋白水解液。结果表明,技术路线Ⅰ、Ⅱ蛋白质回收率分别为86.5%、87.8%;酸溶性氮得率分别为83.2%、86.4%;灰分含量分别为11.1%、7.0%;技术路线Ⅰ所得肽的相对分子质量分布范围为:分子量大于5000的肽占肽总量的20.6%,分子量在3000左右的肽占总量的3.3%,分子量在500～600之间的肽占肽总量的61.3%;技术路线Ⅱ所得的产物组分相对分子质量分布范围为:分子量在1000以下的占71.3%,且分子量主要在700～900之间,分子量在2000左右的占5.7%,分子量在5000以上的占12.8%。研究结果表明,技术路线Ⅱ的酶切效果优于技术路线Ⅰ的。The procedure to obtain oligopeptide with soybean protein isolate by sequential treatment with two proteinases was described. The experiment were designed ： technique one （T1） kept optimum pH of proteinase I for 3h by adding NaOH, technique two （T2） kept optimum pH of proteinase I only at first 30 minutes and hydrolysate are immediately hydrolyzed by proteinase II on acid and neutral condition respectively. The results showed that protein recovery rate are 86.5 %, 87.8 % and acid soluble nitrogen rate are 83.2 %, 86.4 % and ash contents are 11.1% and 7.0% respectively. The molecular weight distribution of peptides obtained by T1 above 5000, about 3000 and between 500 and 600 are 20.6%, 3.3% and 61.3% respectively; The molecular weight distribution of peptides obtained by T2 above 5000, about 2000 and between 700 and 900 are 12.8%, 5.7% and 71.3% respectively. It showed that hydrolysis condition of T2 is better than T1＇s.

关 键 词：碱性丝氨酸蛋白酶 蛋白酶Ⅱ 大豆分离蛋白 寡肽 

分 类 号：Q556.9[生物学—生物化学]