同步辐射对黑曲霉β-葡萄糖苷酶产生条件及酶学性质的影响  

Effects of X-Ray Nk Synchronous Radiation on Production and Characteristics of Aspergillus niger β-glucosidase Mutant

作　　者：潘利华[1] 罗建平[1] 罗水忠[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2008年第5期245-249,共5页Food Science

基　　金：安徽省自然科学基金项目(050410301);合肥工业大学科学发展基金项目(053001F)

摘　　要：本实验对出发菌黑曲霉M85和经软X射线Nk同步辐射所得突变株(产β-葡萄糖苷酶)的营养条件、培养条件和产酶动力学进行了比较,并研究了两菌株β-葡萄糖苷酶的酶学性质。实验结果表明,与出发菌相比,突变株在营养需求、培养条件、产酶动力学和产物的酶学性质都有所改变。突变株的氮源谱更宽,对KH2PO4的需求从0.2%降为0.1%;培养条件实验表明突变株需氧量增加;突变株发酵周期缩短了16h,产酶能力提高了约80%;突变株β-葡萄糖苷酶的最适反应温度从60℃降为50℃,最适反应pH由4升为5。Production and characteristics of two β-glucosidase strains, Aspergillus niger M85 and its mutant obtained by soft X-ray Nk, synchrotron irradiation were studied. The results showed that the nutrient demands, culture condition, β-glucosidase production kinetics and properties of the mutant strain are distinctively changed in comparison with the original strain M85. The spectrum of nitrogen sources of the mutant has been broadened and the demand of KH2PO4 reduced from 0.2% to 0.1%. The mutant was addicted to enough oxygen. The fermentation kinetics test showed that fermentation cycle of the mutant is shortened by 16 hours and the β-glucosidase activity is raised about 80%. The optimal temperature and pH for the mutant β-glucosidase reaction are shifted from 60 ℃ to 50 ℃ and from 4.0 to 5.0, respectively.

关 键 词：黑曲霉 Β-葡萄糖苷酶 软X射线 酶学性质 

分 类 号：Q93[生物学—微生物学]