一株紫杉醇产生菌发酵条件的初步研究  

Preliminary study on fermentation conditions of taxol-producing endophytic fungus

作　　者：代文亮[1] 陶文沂[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《化工进展》2008年第6期883-886,891,共5页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金资助项目(30370044;30470016)

摘　　要：研究了一株内生真菌Fusarium mairei UH23发酵产紫杉醇的工艺条件。实验结果表明最佳培养条件为:温度26℃,发酵起始pH值7.0,接种量5%,装液量250 mL三角瓶装液60 mL,发酵周期12天。筛选出最佳碳源为葡萄糖,最佳氮源为硝酸铵。在上述条件下发酵,紫杉醇产量达(286.4±20.3)μg/L,较优化前提高10.2%。The fermentation conditions of endophytic fungus Fusarium mairei UH23 were investigated for taxol production. The results showed that an inoculation volume of 5% into the 60 mL/250 mL Erlenmeyer flask at 26℃ and initial pH 7.0 could reach the maximum taxol production after 12 days of incubation. Glucose and ammonium nitrate were selected as the best carbon and nitrogen sources in the further study. The taxol yield under the optimized conditions reached （286.4±20.3） μg/L, an increase by 10.2%.

关 键 词：内生真菌 紫杉醇 发酵条件 

分 类 号：TQ460.4[化学工程—制药化工] TQ925[轻工技术与工程—发酵工程]