马铃薯发酵产氢的菌株培养基改良研究  

Improvement of Bacteria Substrate for Hydrogen Production from Potatoes by Anaerobic Fermentation

作　　者：谢斌飞[1] 程军[1] 周俊虎[1] 宋文路[1] 刘建忠[1] 岑可法[1] 

机构地区：[1]浙江大学能源清洁利用国家重点实验室,杭州310027

出　　处：《西安交通大学学报》2008年第6期774-778,共5页Journal of Xi'an Jiaotong University

基　　金：国家自然科学基金资助项目(50406022;50525620);国家高技术研究发展计划资助项目(2006AA05Z122);教育部高等学校全国优秀博士学位论文专项基金资助项目(200437);霍英东高校青年教师基金资助项目(104021);浙江省科技攻关计划资助项目(2006C33049)

摘　　要：为了促进马铃薯发酵产氢,研制了改良培养基驯化产氢菌株并使用淀粉生物酶降解马铃薯.将活性污泥煮沸后作为产氢菌株的接种体富集培养,使用Cl^-代替SO4^2-改良传统培养基消除了传统发酵产氢的启动滞留期（约12 h）,单位最大产氢速率从703.4 mL/（g.d）提高到800.5 mL/（g.d）,最大产氢潜力从205.2 mL/g增加到218.2 mL/g.通过α淀粉酶和糖化酶处理进一步提高了马铃薯的发酵产氢能力,单位最大产氢速率进一步提高到944.7 mL/（g.d）,最大产氢潜力进一步提高到267.2 mL/g.生物气中氢气的体积分数为43%-69%,无甲烷.In order to improve the hydrogen production from potatoes, the bacteria substrate was improved to domesticate H2-producing bacteria and potatoes are degraded by enzyme. The boiled activated sludge was used as H2-producing bacteria inoculum. While using the improved substrate, in which Cl^- was used to substitute for SO4^2-, a lag-phase time （about 12 h） vanished, the maximum specific hydrogen production rate increased from 703. 4 mL/（g · d） to 800.5 mL/ （g · d） and the maximum hydrogen yield increased from 205.2 mL/g to 218. 2 mL/g. When potatoes were pretreated by a amylase and glucoamylase, the hydrogen production was further enhanced. The maximum specific hydrogen production rate was then increased to 944.7 mL/（g · d） and the maximum hydrogen yield was increased to 267.2 mL/g. It is found that the hydrogen composition of the biogas is about 43%-69% and no methane is detected.

关 键 词：产氢 发酵 培养基 酶解 

分 类 号：X172[环境科学与工程—环境科学]