茶叶多酚氧化酶的序列分析与结构预测  

A Sequence Analysis and Structural Prediction on Polyphenol Oxidase in Tea

作　　者：潘宇[1] 王坤波[1] 徐仲溪[1] 徐辉[2] 何志丹[1] 

机构地区：[1]湖南农业大学茶学教育部重点实验室 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《茶叶科学》2008年第3期157-165,共9页Journal of Tea Science

基　　金：湖南科技厅[湘科计字(2007)109]

摘　　要：调用生物信息学方法对已在国际互联网上的生物数据库(DDBJ、Uni-Prot)注册的茶树多酚氧化酶基因的核酸及氨基酸序列进行分析,并对其组成成分、信号肽、跨膜拓扑结构域、疏水性/亲水性、蛋白质二级及三级结构进行预测与推断。结果表明:茶多酚氧化酶不具有信号肽,存在卷曲螺旋,为位于细胞质中的无跨膜的亲水性不稳定蛋白,无规则卷曲是其蛋白质结构的主要结构元件,α-螺旋与β-折叠分散于整个多肽链中,并存在2个结构功能域。In the present study, Polyphenol Oxidase （PPO） in tea plant which was registered in biodatabase （DDBJ ＆ Uni-Prot） were analyzed and predicted by method of bioinformatics, including composition of nuclei acid sequences and amino acid sequences, signal peptide, transmembrane topological structure, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein. The results showed that PPO in tea was a hydrophilic and non-transmembrane protein without signal peptide, but had coils. The main motifs of its predicted secondary structure of had random coil, alpha helix and beta turn were spread to the whole secondary structure of protein. Its amino acid sequences included two functional domains.

关 键 词：茶树 多酚氧化酶 序列分析 结构预测 

分 类 号：S571.1[农业科学—茶叶生产加工] Q523[农业科学—作物学]