雪灵芝水溶性多糖提取工艺优化及其数学模型的建立  

Optimization of the Extraction Process of Water-soluble Polysaccharides of Arenaria Kansuensis Maxim and the Establishment of Its Mathematical Model

作　　者：朱影[1] 旺梅 马荣池[1] 许倩兮[1] 吕静[1] 彭光华[1] 

机构地区：[1]华中农业大学食品科学技术学院,武汉430070 [2]西藏自治区质量技术监督局,拉萨850000

出　　处：《世界科技研究与发展》2008年第3期249-252,共4页World Sci-Tech R&D

摘　　要：目的优化雪灵芝水溶性多糖的提取工艺，并建立数学模型。方法采用水提醇沉法提取雪灵芝水溶性多糖；运用苯酚-硫酸法测定多糖含量；通过单因素实验研究浸提时间、加水比、浸提温度及乙醇浓度对多糖提取率的影响；采用响应面分析法优化提取条件，运用sas软件分析实验数据，建立回归模型。结果得到雪灵芝水溶性多糖提取的优化工艺参数的最佳取值范围为：浸提温度（94．5～97．5℃）；浸提时间（2～2．5h）；加水比（43～51v／w）。结论为雪灵芝水溶性多糖提取工艺的优化提供了一定的技术依据，也为其后续研究奠定了基础。Objective To optimize the extraction process of water - soluble polysaccharides of Arenaria kansuensis Maxim, and establish math- ematical models, Methods The polysaeeharides were extracted with hot water, then isolated by ethanol ; Using phenol - sulfate method determinate polysaccharides＇ content; The effect of extraction time, extraction proportion of solvent and stuff, extraction temperature and the concentration of ethanol on the extraction rate was studied by single factor experiment ; Extraction conditions were optimized by Response Surface Analysis ; Experimental data analyses and the establishment of the regression models was carried out by Sas-software. Results The best value ranges of the parameters in the Optimized extraction process are ： extraction temperature （ 94.5 - 97.5 ℃ ） ; extraction time （ 2 - 2.5 h） ; extraction proportion of solvent and stuff （43 -51 v/w）. Conclusion Provide a certain technical basis for optimizing the extraction process of water - soluble polysaccharides of Arenaria kansuensis Maxim, also laid a foundation for the follow-up study.

关 键 词：水溶性多糖 提取工艺 响应面分析 

分 类 号：TQ461[化学工程—制药化工]