液质联用测定薯蔓中黄酮类化合物的构成  

Component Analysis of Flavonoids from Sweet Potato Vines by HPLC-MS

作　　者：罗丽萍[1,2] 高荫榆[1,2] 洪雪娥[1] 夏冬华[1] 

机构地区：[1]食品科学与技术国家重点实验室(南昌大学),江西南昌330047 [2]生物质转化教育部工程研究中心,江西南昌330047

出　　处：《食品科学》2008年第6期52-55,共4页Food Science

基　　金：江西省科技厅农业重大攻关项目(2003CB415100)

摘　　要：以我国广泛栽培的徐薯18薯蔓为原料,中试设备水提多糖后的滤渣经醇提、AB-8树脂精制获得薯蔓黄酮(FSPV)。利用HPLC-ESI-MS对FSPV进行分析,从获得的质谱分子量信息初步鉴定出了6个主要的黄酮甙元。进一步结合HPLC,通过与标样保留时间对比,确定FSPV中存在:染料木素、木犀草素、槲皮素、刺芒柄花素、芹菜素、山奈酚6种黄酮甙元。其中,首次报道薯蔓中存在染料木素和刺芒柄花素。To exploit the rich resources, sweet potato vines （SPV） of Xushu 18 were selected as raw materials since this kind was cultivated in China widely. The remains of the SPV after extracting polysaccharides by hot water were used to extract flavanoid by ethanol in pilot instrument. Then flavonoid from sweet potato vines （FSPV） were purified and obtained by AB-8 resin. The kinds of flavonoid aglycones in FSPV were identified by the molecular weight information from HPLC-ESI-MS. Comparing the retention time of FSPV with those of standard samples, it was determined that there are six aglycones in FSPV. They are genistein, leteolin, quercetin, formononetin, apigenin and kaempferol respetively. This is the first time to report genistein and formononetin existing in SPV.

关 键 词：高压液相色谱-电喷雾离子阱质谱 高压液相色谱 构成 黄酮类化合物 薯蔓 

分 类 号：TS207.3[轻工技术与工程—食品科学]