从红曲霉菌丝体中提取红色素的工艺  

On fermentation and extraction craft of Monascus pigment

作　　者：林元山[1] 詹逸舒[1] 梁智群[2] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]广西大学生命科学院,广西南宁530004

出　　处：《湖南农业大学学报（自然科学版）》2008年第3期355-358,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(20066001)

摘　　要：红曲霉Monascus SP4-2经10 L发酵罐小试液体发酵、过滤、洗涤,获得菌丝体.在通过单因素试验研究提取次数,提取剂浓度、用量,浸提温度,浸提时间对红曲色素提取影响的基础上,采用正交试验研究了提取剂浓度、浸提时间、浸提次数对红曲色素提取的影响.试验结果表明,从干红曲霉菌丝体中提取红色素的最佳工艺条件为:以90%乙醇为提取剂,提取温度40℃,提取时间40 min,在此条件下提取3次可使菌丝体中红色素的提取率高达96.7%.Mycelia of Monascus SP4-2 were prepared by filtration and washing after fermentation in 10 L glass fermentator. Extraction craft of Monascus pigment from mycelia were performed by methods of single factor and orthogonal design experiments. The results showed that optimum processing Monascus pigment of mycelia were under conditions of alcohol 90%, 40 ℃, 40 minutes every time for three times of extraction and the total ratio of Monascus pigment of mycelia reached 96.7%.

关 键 词：红曲霉 红曲红色素 提取工艺 

分 类 号：TS264.4[轻工技术与工程—发酵工程]