不同诱变方法对青霉TS406Y胆固醇氧化酶活性的影响  

Effect on the Activity of Cholesterol Oxidase by Different Mutation Methods

作　　者：王素英[1] 高朋辉[1] 马熟军[1] 

机构地区：[1]天津商业大学食品生物技术重点实验室,天津300134

出　　处：《河北师范大学学报（自然科学版）》2008年第4期534-537,共4页Journal of Hebei Normal University：Natural Science

基　　金：天津市自然科学基金(05YFJMJC06100)

摘　　要：以青霉TS406Y(Penicillum sp.)为诱变出发菌株,评价了硫酸二乙酯(DES)、亚硝基胍(NTG)和甲基磺酸乙酯(EMS)对该菌株的诱变效应,发现在常规剂量内达到70%致死率时所需要的时间分别为23,3,90 min.根据使用方便和安全原则,进行了青霉TS406Y的DES诱变和选育,获得了胆固醇氧化酶活性提高84.5%的突变菌株D5,其胆固醇氧化酶活性达到了1240 U/L,连续传代6次,发现D5菌株具有遗传稳定性.另外还发现,利用紫外线(UV)和EDS对单因素诱变后获得的胆固醇氧化酶高产菌株进行复合诱变,94%的突变菌株为负变菌株.Mutated effect was determined of mutagenic agent which were diethyl sulfate, N-Methyl-N-ni- troso-N＇-nitroguanidine and Methanesulfonic acid ethylester, when the Penicillum sp. TS406Y was treated for 23,3,90 min by above mutagenic agent respectively, 70 % lethal rate was gotten. Cholesterol-degrading mutant D5 was obtained from Penicillum sp. TS406Y by treatment with diethyl sulfate. The activity of cholesterol oxidase of strain DS, which was 1 240 U/L, and was 84.5 % higher than TS406Y. Meanwhile the results showed that 94 % negative mutants were obtained by the combined treatment with ultraviolet and diethyl sulfate.

关 键 词：胆固醇氧化酶 诱变育种 青霉TS406Y 发酵 

分 类 号：Q933[生物学—微生物学]