酸性木聚糖酶产生菌产酶条件及部分酶学性质研究  

Study on Production and Part Enzymology Characterization of Acid-stable Xylanase Enzyme from Bacteria

作　　者：濮智颖[1] 

机构地区：[1]陕西教育学院生命科学系,陕西西安710061

出　　处：《陕西教育学院学报》2008年第2期101-105,共5页Journal of Shaanxi Institute of Education

摘　　要：从煤矿酸性废水中分离到一株产木聚糖酶青霉,通过酸性液体培养研究了菌体生长对pH的响应及木聚糖酶的产生特征,并测定了木聚糖酶的部分应用性质。结果表明:实验菌株嗜酸,菌丝生长最适pH为2.0,孢子萌发生长适宜pH为3.0-4.0;木聚糖诱导菌体在生长稳定期大量产生木聚糖酶,蛋白胨是菌体产酶的适宜氮源;菌株所产木聚糖酶属于酸性木聚糖酶,反应最适pH3.5、最适温度50℃-55℃,在最适反应条件下保温60min,残余酶活仍接近70%,适用于较强酸性的高温加工环境。Penicillium acidophilus isolated from coal mine＇s acidic wastewater can produce acid-stable xylanase in liquid culture. Both the growth pH and characteristics of the production and xylanase from this strain were investgated. The results show that this strain was a typical acidophile with optimal growth pH being 2.0 for hypha and pH 3.0 - 4.0 for the spore germination. Peptone was the most suitable nitrogen source which helped produce xylanase induced by xylan during stationary phase. The resultant xylanase reacted best at 50℃ - 55℃ and pH 3.5, and had activities 70 % after 60 min maintenance under optimum reaction conditions.

关 键 词：木聚糖酶 营养条件 酶学性质 嗜酸菌 

分 类 号：Q532.2[生物学—生物化学]