破壁蛋白核小球藻对小鼠免疫调节作用  

Study on immunoregulation of chlorella pyrenoidos with disintegrated cell wall on mice

作　　者：封会茹[1] 计融[2] 李燕俊[2] 李业鹏[2] 江涛[2] 赵熙[2] 钟凯[2] 韩春卉[2] 李玉伟[2] 张靖[2] 陈庭君[2] 

机构地区：[1]北京市丰台区疾病预防控制中心微生物检验科,北京100071 [2]中国疾病预防控制中心营养与食品安全所

出　　处：《中国公共卫生》2008年第7期836-837,共2页Chinese Journal of Public Health

基　　金：卫生部法监司资助项目“保健食品功能学评价程序和检验方法研究”(2002-2003)

摘　　要：目的探讨破壁蛋白核小球藻对健康小鼠免疫系统的影响。方法选用BalB/c正常小鼠,随机分组,经口给予小鼠不同剂量的小球藻粉水溶液0,0.15,1.50,4.50g/(kg.bw)每日1次,30 d后测量体重增长值、胸腺体重比值、脾脏体重比值、迟发型变态反应、刀豆蛋白A(ConA)诱导的小鼠淋巴细胞转化、抗体生成细胞数、半数溶血值、小鼠碳廓清能力、小鼠腹腔巨噬细胞吞噬鸡红细胞能力、杀伤细胞(NK)活性。结果破壁蛋白核小球藻能促进小鼠体重增长,提高小鼠的脾脏体重比值,增强小鼠的迟发型变态反应能力,增强ConA诱导的小鼠淋巴细胞转化能力,增强巨噬细胞吞噬鸡红细胞的能力,增强NK细胞活性,对胸腺体重比值、抗体生成细胞数、半数溶血值、单核-巨噬细胞碳廓清能力无显著影响。结论破壁蛋白核小球藻对健康小鼠具有免疫调节作用。Objective To study the influence of chlorella on the immune system of healthy mice. Methods 60 BALB/c mice were selected and divided randomly into four groups. Different doses of chlorella pyrenoidosa[ 0, 0.15, 1.50, 4.50g/（ kg· bw） ] were administered to the mice orally for 30 days. A series of classical tests were adopted to evaluate the effects. Results The weight gain of the mice administered with chlorella pyrenoidosa was higher than that of the control, and the difference has statistical significance（ P 〈 0.05 ）. The ratio of spleen/body weight, delayed hypersensitivity response, the lymphocyte transformation of mice induced by conA, the phagocytotic ability of macrophages, NK cell activity were all higher than those of the control and had statistically significant differences（ P〈 0.05 ）. The ratio of thymus gland/body weight, the number of antibody - producing cells, the amount of hemolytic antibody and the phagocytotic ability of macrophages for chicken erythrocytes were higher than those of the control, too. But there was no statistically significant difference（P〈0.05）. Conclusion Chlorella pyrenoidosa with disintegrated cell wall can modulate the immune system of healthy mice.

关 键 词：小球藻 免疫调节 细胞免疫 体液免疫 单核-巨噬细胞 NK细胞活性 

分 类 号：R151.1[医药卫生—营养与食品卫生学] R979.5[医药卫生—公共卫生与预防医学]