胰腺癌细胞基因组范围内的基因缺失  

Genome-wide gene loss in human pancreatic cancer cells

作　　者：林连捷[1] 王玉峰[2] 郑长青[1] 金玉[1] 胡刚正[1] 刘香[1] 林艳[1] 

机构地区：[1]中国医科大学附属盛京医院消化内科,辽宁省沈阳市110004 [2]沈阳二四五医院药剂科,辽宁省沈阳市110042

出　　处：《世界华人消化杂志》2008年第17期1849-1854,共6页World Chinese Journal of Digestology

摘　　要：目的:研究胰腺癌细胞基因组范围内的纯合性缺失和杂合性缺失(loss of heterozygosity,LOH).方法:应用高密度单核苷酸多态性芯片和专用分析软件,检测17种胰腺癌细胞株基因组范围内的纯合性缺失和LOH,并筛选可能与胰腺癌发生、发展有关的基因区域,用PCR验证纯合性缺失.结果:经过PCR验证,26个区域确实为纯合性缺失,芯片的准确度为83.9%(26/31).这些缺失区域中,平均每个区域只涉及1.29个基因.每一种细胞都有不同程度的LOH;不同染色体臂出现LOH频率不同,出现频率最高的为染色体臂9p和18q,均为94.1%.结论:胰腺癌全基因组范围内出现多处LOH和纯合性缺失,这些区域可能含有新抑癌基因.AIM： To investigate genome-wide loss of heterozygosity （LOH） and homozygous deletion in human pancreatic cancer cell lines. METHODS： Genome-wide LOH and homozygous deletion in 17 pancreatic cancer cell lines were studied using high-density single nucleotide polymorphism array and the data were analyzed using a special analytical software. PCR was performed to verify homozygous deletion following screening for potential genetic domains associated with development of pancreatic cancer. RESULTS： A total of 26 homozygous deletions were verified by PCR and the accuracy of the chip was 83.9% （26/31）. On average, 1.29 genes were involved in each region. Each pancreatic cancer cell line had different LOH. Different chromosome arms presented with various LOH frequency with the most common abnormalities in 9p and 18q, occurring in 16 cell lines （94.1%）. CONCLUSION： Genome-wide LOH and homozygous deletions are common in pancreatic cancer cell lines, indicating existence of novel tumor suppressor genes.

关 键 词：单核苷酸多态性芯片 杂合性缺失 纯合性缺失 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]