牛乳铁素的制备纯化与氨基酸序列分析  

Preparation and Purification and Amino Acid Sequence Analysis of Bovine Lactoferricin

作　　者：胡志和[1] 冯飞[1] 刘传国[1] 庞广昌[1] 

机构地区：[1]天津市食品生物技术重点实验室,天津商业大学食品科学与工程系,天津300134

出　　处：《食品科学》2008年第7期166-170,共5页Food Science

基　　金：天津市重点科技支撑项目(07ZCKFC00200)

摘　　要：对牛乳铁素的制备和分离纯化方法进行研究。牛乳铁蛋白经胃蛋白酶水解,其产物经不同分离介质进行分离纯化,用毛细管电泳检测其纯度。用氨基酸分析仪确定其氨基酸组成,并进行适合度检验。结果表明,采用Butyl-Toyopearl650M树脂分离,其纯度为95.6%。纯化出的产物氨基酸组成和序列与已知的牛乳铁素无显著差异。The method of preparation and Isolation and purification of bovine lactoferricin（LfcinB） was studied. Hydrolysate of lactoferrin by pepsin was separated and purified by different medium, and the purity of lactofrricin was detected by capillary electrophoresis method. The amino acid compose of lactoferricin was analyzed by automatic amino acid analyzer, and the amino acid sequence was confirmed by the method of goodness of fit test. The results showed that preparation purity of lactoferrcin by Butyl-Toyopearl 650M is 95.6%. The amino acid compose and sequnce of the purification production there is no significant difference comparison for known LfcinB.

关 键 词：乳铁蛋白 乳铁素 分离纯化 氨基酸序列分析 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]