贮藏苹果中展青霉素产生菌的分离及其ITS序列分析鉴定  

Isolation of main patulin-producing strains from storage apples and their ITS sequences analysis and identification

作　　者：刘盼红[1] 岳田利[1] 袁亚宏[1] 高振鹏[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第8期197-202,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十一五"科技支撑计划项目(2006BAK02A24;2006BAK02A18;2006BAK04A05);陕西省重大科技专项计划项目(2006KZ09-G1);教育部新世纪优秀人才支持计划项目(2005)

摘　　要：【目的】分离贮藏苹果中的展青霉素产生菌,并对其ITS序列进行分析鉴定。【方法】从贮藏库中苹果上分离展青霉素产生菌,采用真菌ITS区特异性引物对其ITS区进行PCR扩增测序,将分离菌ITS区序列与GenBank中的核酸数据库进行比对分析,确定展青霉素产生菌的生物学分类,并对其亲缘关系进行系统发育分析。【结果】共分离得到10株展青霉素产生菌,其中1号和8号菌株属曲霉属,其他8株属青霉属。在系统发育关系上,1号和8号菌为一个类群,其他8株菌属于一个类群。【结论】贮藏苹果中分离得到的10株展青霉素产生菌为青霉属或曲霉属菌株,它们之间存在着不同程度的亲缘关系。[Objective] This study isolated main patulin-producing strains from storage apples,analyzed and identified their ITS sequences. [Method] In this paper patulin-producing strains were isolated from storage apples and the fungal ITS region-specific primers were used to do ITS PCR amplification sequencing, through the blast analysis between ITS sequences and GenBank nucleic acid database, finally their biological classifications were identified and their relationships were analyzed. [Result] ①10 patulin-producing strains were isolated from storage apples. ②Strain 1 and 8 are Aspergillus,and the remaining eight strains were Penicillium.③The phylogenetic tree showed that strain 1 and 8 belonged to one group, the other eight strains to another group. [Conclusion] 10 patulin-producing strains from storage apples are Aspergillus or Penicillium and they have different levels of the pbylogenetic relationship.

关 键 词：苹果 展青霉素 展青霉素产生菌 ITS 

分 类 号：TS201.3[轻工技术与工程—食品科学]