安络小皮伞胞外及胞内多糖体外抗氧化性的研究  

STUDY ON IN VITRO ANTI-OXIDATION OF MARASMIUS ANDROSACEUSEXTRACELLULAR POLYSACCHARIDES

作　　者：董媛[1] 高翔[1] 李婷婷[1] 赵兴红[1] 滕利荣[1] 孟庆繁[1] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《食品研究与开发》2008年第4期45-48,共4页Food Research and Development

基　　金：吉林大学国家级生物实验教学示范中心设计创新实验基金二类资助项目(ZXCX2006009)

摘　　要：为了充分利用安络小皮伞菌资源,通过液体深层发酵获得安络小皮伞菌丝体及发酵滤液,采用水提醇沉法提取菌丝体胞内多糖,采用醇沉法提取发酵滤液胞外多糖。通过体外实验研究安络小皮伞胞外及胞内多糖对羟自由基、超氧阴离子、DPPH的清除作用以及对H2O2诱导的红细胞氧化溶血和大鼠肝匀浆脂质过氧化的抑制作用。结果表明,胞外、胞内多糖对羟自由基均具有显著的清除作用,对超氧阴离子、DPPH的清除作用以及对大鼠肝匀浆脂质过氧化的抑制作用良好,对H2O2诱导的红细胞氧化溶血抑制作用较弱,相同浓度时胞内多糖的抗氧化活性高于胞外多糖。To make the best use ofMarasmius androsaceus sources. Marasmius androsaceus mycelium and fermented filtrated liquor were acquired by means of deep liquid fermentation. Water-extraction and ethanol-precipitation method was used to extract intracellular polysaccharides in mycelium, and ethanol-precipitation method was used to extract extracellular polysaccharides in fermented filtrated liquor. The in vitro scavenging activities of polysaccharides on hydroxyl radical,superoxide radical,and DPPH, and its inhibitory effects on H2O2-induced hemolysis of rat erythrocytes and lipid peroxidation of liver homogenate in rat were investigated. The results of anti-oxidative experiments showed that the elimination rate of extracellular and intracellular polysaccharides to ·OH were pretty high. Their effects on ·O2-, DPPH and lipid peroxidation of liver homogenate in rat were evident. They had less influence on H2O2-induced hemolysis of rat erythrocytes. With the same concentration, intracellular polysaccharides were more effective than extracellular polysaccharides on anti-oxidation.

关 键 词：安络小皮伞 胞外多糖 胞内多糖 体外抗氧化性 

分 类 号：R284.1[医药卫生—中药学]