响应曲面优化酶法制备蛹虫草抗氧化活性成分的研究  

OPTIMIZATION OF ANTIOXIDANT ACTIVE COMPONENTS DERIVED FROM CORDYCEPS MILITARIS WITH NEUTRASE USING RESPONSE SURFACE METHODOLOGY

作　　者：韩晨[1] 刘春泉[1] 玄国东[1] 贺稚非[2] 

机构地区：[1]江苏省农业科学院原子能农业利用研究所,江苏南京210014 [2]西南大学食品科学学院,重庆400716

出　　处：《食品研究与开发》2008年第4期83-87,共5页Food Research and Development

摘　　要：研究了蛋白酶解法制备蛹虫草抗氧化活性成分的工艺。运用响应曲面实验设计(RSM),通过部分因子设计(FFD)、爬坡实验设计、中心点实验设计(CCD)三部分实验优化了酶解工艺参数。研究表明,pH=6.5,温度45℃,中性蛋白酶添加量(E/S)7.03%,液固比(W/R)25.41,反应时间4h时,蛹虫草酶解液的还原力A700达到0.465。The effect of process conditions on antioxidant activity of Cordyceps militaris hydrolyzed with Neutrase has been investigated using response surface methodology （RSM） in this paper. RSM was including three parts： Fractional factorial design（FFD）, steepest ascent design, and central composite design（CCD）.The optimum conditions of enzymatic hydrolysis were： 6.5 pH value, 45℃ temperature, 7.03 % ratio of enzyme and substrate, 25.41 ratio of water and raw material, 4 h time, the predicted value at stationary point of reducing power（A700） was 0.465.

关 键 词：蛹虫草 抗氧化活性 蛋白酶 响应曲面法 

分 类 号：R284[医药卫生—中药学]