4种富硒植物预防N-甲基-N’-硝基-N-亚硝基胍诱发大鼠胃癌效果研究 三、不同源硒预防实验性胃癌大鼠组织内硒累积分布研究  

Preventive effects of 4 Se-enriched plants on rat stomach cancer induced by MNNG 3.Se accumulation and distribution in rats of different selenium resources for prevention of stomach cancer

作　　者：杨文婕[1] 陈竞[1] 李卫东[1] 陈晓滨[1] 曲宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2008年第4期435-437,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No30170800)

摘　　要：目的对比研究长期摄入高剂量不同富硒植物对大鼠体内硒累积分布的影响。方法采用N-甲基-N’-硝基-N-亚硝基胍(MNNG)诱发大鼠胃癌模型,连续灌以硒高剂量四种不同富硒植物17周,测定大鼠硒累积以及参与硒蛋白合成组织的含硒量。结果摄入富硒青花菜、富硒红羽、富硒绿羽的大鼠肝和肾硒水平显著高于摄入富硒大蒜的,摄入富硒大蒜的大鼠红血球和脾脏硒水平显著高于摄入富硒青花菜、富硒红羽和富硒绿羽的。结论初步认为长期摄入高剂量植物硒后,动物组织硒的累积和分布取决于摄入的硒种类;与其它源硒相比,长期摄入高剂量富硒大蒜后,动物肝组织的低硒累积可以作为富硒大蒜高安全性的重要依据之一。Objective To investigate the accumulation and distribution of blood and tissue Se in rats with long-term and high-dose of Se supplementation by four Se-enriched plants （Se-enriched garlic, Se-enriched broccoli, Se-enriched green kale and Se-enriched red kale） in the N-methyl-N＇-nitro-N-nitrosoguanidine （MNNG）-induced stomach cancer model. Methods Ninety rats were fed by basal diet for a week, divided equally into the control, MNNG and seven Se supplementation groups. Rats were daily given 15mg/kg bw of MNNG for ten days except those in the control group, and rats in five Se-enriched plant treatment groups were daily given 150 or 300μgSe/kg bw of the plant suspension by gavage for 17 weeks. Rats were sacrificed at the end of 18th week, the Se contents of blood, red blood cell, plasma, liver, kidney, spleen, heart, brain and testicle were determined. Results The Se contents of liver and kidney in rats supplemented by Se-enriched garlic were significantly lower than those in rats supplemented by Se-enriched broccoli and two Se-enriched kales, and the Se contents of red blood cell and spleen higher than those in rats supplemented by Se-enriched broccoli and two Se-enriched kales. Conclusion The data primarily shows that the differences of the accumulation and distribution of blood and tissue Se in rats are related to the supplemented plant Se components, and that lower Se accumulation of the liver and kidney in rats supplemented by Se-enriched garlic than by other plants may be one of Se safety indices of Se-enriched garlic.

关 键 词：富硒植物 MNNG胃癌模型 硒累积分布 胃癌 微量元素 

分 类 号：Q581[生物学—生物化学] R735.2[医药卫生—肿瘤]