野生牛肝菌多糖的分离鉴定及其抗氧化性研究  

Separation and Identification of Polysaccharides from Natural Boletus and Their Antixidant Activities

作　　者：杨立红[1] 刘林德[1] 钟旭生[1] 冯培勇[1] 蔡德华[1] 马崇军[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《食品科学》2008年第8期335-338,共4页Food Science

基　　金：山东省教育厅科技计划项目(J06NO2)

摘　　要：野生牛肝菌烘干后经热水浸提,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后上DEAE-纤维素柱层析,分离得到四种多糖组分Nb-Ⅰ、Nb-Ⅱ、Nb-Ⅲ和Nb-Ⅳ。红外光谱对Nb-Ⅰ分析表明:Nb-Ⅰ为含有葡萄糖醛酸的β-D-吡喃葡聚糖。Sepharose CL-4B测得Nb-Ⅰ分子量为4.0×104,总抗氧化能力测定结果表明:牛肝菌粗多糖和Nb-Ⅰ均具有抗氧化能力,且后者高于前者。The crude polysaccharides of wild Natural Boletus were extracted by hot water and deposited by ethanol. DEAE Cellulose column chromatography was used for polysaccharides purification after the protein was removed by Seveg method, and four kinds of polysaccharides named as Nb-Ⅰ, Nb-Ⅱ, Nb-Ⅲand Nb- Ⅳ were separated. Infrared spectrum analysis showed that Nb-Iis β-D- glucopyranose containing glucuronic acid, and Sepharose CL-4B method showed that molecular weight of Nb- Ⅰ is 4.0 ×10^4. Both crude polysaccharides and Nb- Ⅰ have total antioxidafion acfivity（T-AOC）, and that of Nb- Ⅰ is higher.

关 键 词：野生牛肝菌 多糖 分离鉴定 总抗氧化性 

分 类 号：Q949.3[生物学—植物学]