HPLC-ESI-MS／MS测定动物性食品中19种喹诺酮类药物残留的研究  

Simultaneous and High Sensitivity Determination of Nineteen Kinds of Quinolone Multiresidues in Animal Food by HPLC-ESI-MS/MS

作　　者：李雅丽[1] 郝晓蕾[1] 冀宝庆[1] 胥传来[1] 沈崇钰[2] 丁涛[2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江苏省出入境检验检疫局,江苏南京210001

出　　处：《食品科学》2008年第8期502-506,共5页Food Science

基　　金：“十一五”国家科技支撑计划重点项目(2006BAK02A09)

摘　　要：本研究建立了同时检测动物性食品中恩诺沙星、环丙沙星、诺氟沙星、氧氟沙星、氟甲喹、恶喹酸、双氟沙星、沙拉沙星、司帕沙星、丹诺沙星、氟罗沙星、马波沙星、伊诺沙星、奥比沙星、培氟沙星、萘啶酸、吡哌酸、洛美沙星和西诺沙星19种喹诺酮类药物的HPLC-ESI-MS/MS检测方法。动物组织样品用乙腈提取,采用甲酸水溶液-甲醇体系作为流动相,梯度洗脱,质谱检测器检测。方法的线性范围为0.3～50μg/kg,相关系数(r)大于0.9956,检出限为0.3μg/kg,19种喹诺酮类药物在鸡肉和鱼肉的平均回收率分别为75.3%～96.3%、79.7%～94.2%,日内和日间变异系数均小于10%。A multi-residue method was developed for the confirmation and quantitation of nineteen kinds of quinolones in animal food by HPLC-ESI-MS/MS. The samples were extracted with acetonitrile. The analytes were separated using 0.1% fomic acidmethanol system as the mobile phase with a linear gradient elution program, and determined by tandem mass detector. Results showed that their linear ranges are 0.3 μg/kg to 50 μg/kg with correlation coefficients （r） more than 0.9956, and the limits of detection are 0.3 μg/kg. The mean recoveries for each analyte in chicken and fish range from 75.3%~96.3% and 79.7%~94.2% with relative standard deviations below 10%. The method is convenient, fast, safe, sensitive and accurate, and can be used to simultaneously analyze residual quinolones in actual samples.

关 键 词：喹诺酮类药物 动物性食品 HPLC-ESI-MS/MS 多残留检测 

分 类 号：TS207.53[轻工技术与工程—食品科学]