少根根霉利用木糖和葡萄糖分步发酵制备富马酸  

Stepped Utilization of Xylose and Glucose in Fermentation of Fumaric Acid by Rhizopus arrhizus

作　　者：刘宁[1] 李霜[1] 何皓[1] 吴华[1] 黄和[1] 嵇松杨 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009 [2]北京盈信阳光生物技术有限公司,北京100086

出　　处：《过程工程学报》2008年第4期794-797,共4页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20576054);国家高技术研究发展计划(863)基金资助项目(编号:2006AA02Z240);国家重点基础研究发展规划(973)基金资助项目(编号2007CB707805)

摘　　要：将木糖与葡萄糖分别用于少根根霉种子培养及发酵产富马酸2个阶段,少根根霉利用木糖进行种子培养时,最适木糖浓度为30g/L,摇瓶最适孢子浓度为(4～6)×105mL-1,最佳种龄32～40h,最适接种量为8%(φ),在含有100g/L葡萄糖的产酸发酵培养基中发酵72h后,富马酸最高浓度达53.51g/L.研究结果表明,可以利用木糖替代葡萄糖进行少根根霉的种子培养,分步发酵制备富马酸.The fumaric acid production by Rhizopus arrhizus using xylose as the carbon source of seed culture medium and glucose as carbon source of fermentation medium was studied. In the seed culture, the biomass of Rhizopus arrhizus obtained using xylose was more than that using glucose. The conditions of the seed culture were optimized. The results showed that the optimal xylose concentration was 30 g/L, the suitable spore concentration range （4-6）×10^5 mL^-1 and the seed age range 32-40 h. The final concentration of fumaric acid was 53.51 g/L in the fermentation medium containing 100 g/L glucose. The result implies that the problem of low conversion rate of xylose could be avoided, and the availability of xylose improved, which would facilitate further study on the stepped utilization of lignocellulose hydrolysate in fermentation of fumaric acid.

关 键 词：富马酸 木糖 葡萄糖 少根根霉 分步发酵 

分 类 号：TQ92[轻工技术与工程—发酵工程]