富硒鸡腿菇菌丝体深层发酵培养基的优化  

Optimization of Fermentation Liquid Medium for the Se-Accumulation of Coprinus comatus

作　　者：余杰[1] 崔鹏举[1] 崔仕超[1] 赖晓[1] 李值[1] 

机构地区：[1]汕头大学生物学系,广东汕头515063

出　　处：《汕头大学学报（自然科学版）》2008年第3期45-50,共6页Journal of Shantou University：Natural Science Edition

摘　　要：研究营养因子对鸡腿菇菌丝体生长和富硒的影响,筛选到适合鸡腿菇富硒深层发酵的优化培养基配方为:葡萄糖2%,玉米粉4%,花生粕0.4%,KH2PO4 0.2%,MgSO4·7H2O0.1%,VB1 10×10-3 g/L,Na2SeO3 4.4×10-3 g/L.在此优化培养基的基础上进行5 d的摇瓶发酵培养,得到菌丝体生物量为18.14 g/L,富硒率为56.35%.The effects of the nutrition factor on the biomass and Se-accumulation of Coprinus comatus mycelia were studied. An optimum medium was selected through orthogonal test. The medium composition was as follows： 2.0% glucose, 4% maize power, 0.4% peanut meal, 0.2% KH2PO4, 0.1% MgSO4·7H2O, 10×10^-3g/L VB1, 4.4× 10^-3 g/L Na2SeO3, The results showed that under these conditions, the production of mycelia biomass and the ratio of Se-accumulation was 18.14g/L and 56.35% respectively, when the mycelia had been cultured about 5 d in the shaking flask.

关 键 词：鸡腿菇 深层培养 富硒 

分 类 号：S646.19[农业科学—蔬菜学]