鸡腿菇菌丝深层培养富硒的研究  

A Study on the Se-Accumulation of Coprinus comatus Mycelia in Submerged Culture

作　　者：余杰[1] 崔鹏举[1] 崔仕超[1] 王静仁[1] 

机构地区：[1]汕头大学生物学系

出　　处：《食品与发酵工业》2008年第7期93-97,共5页Food and Fermentation Industries

摘　　要：通过正交试验,从4株鸡腿菇菌株中筛选出1株具有较高生物量和较强富硒能力的鸡腿菇菌株CcⅢ,并探讨了其耐硒和富硒特性。结果表明,CcⅢ菌株具有一定的耐硒能力和很强的富硒能力,其固态培养最大耐硒浓度为10μg/mL,液态培养最大耐硒浓度为4μg/mL。在硒浓度2μg/mL时,菌丝体生物量为4.11 g/L,富硒量246.74μg/g,富硒率为50.57%,有机化程度高达92.95%。In this paper, the mycelia biomass and the ability of selenium （Se） accumulation of four strains of Coprinus comatus were firstly investigated. Although the strain of Coprinus comatus Ⅲ did not show the highest production of mycelia biomass, it showed the highest ability of Se accumulation according to the orthogonal experimental design. Thus Coprinus comatus Ⅲ was selected to test the characters of Se tolerance and accumulation. The result showed that the production of mycelia biomass and Se-accumulation was 4.11g/L and 246.74 μg/g espectively when the concentration of Se is 2 μg/mL while the ratio of Se accumulation and organic Se was 50.57% and 92.95% respectively under the same condition. This result provides a new way for the Chinese people to resolve the shortage of Se uptake.

关 键 词：鸡腿菇 深层培养 富硒 

分 类 号：S646[农业科学—蔬菜学]