细菌纤维素耐低温生产菌株的诱变选育  

Mutation and selection of low temperature-resistant bacteria for cellulose production

作　　者：王海波[1] 马霞[1] 王腾飞[1] 任尚美[1] 关凤梅[1] 

机构地区：[1]山东轻工业学院食品与生物工程系,山东济南250353

出　　处：《中国酿造》2008年第9期10-12,共3页China Brewing

基　　金：山东省自然科学基金(Q2006F08);上海市博士后基金(06R214106)

摘　　要：以实验室保存的木醋杆菌(Acetobacterxylinum)为出发菌株,利用紫外诱变的方法,以获取在低温条件下能高产细菌纤维素的突变株。通过初筛和复筛以及连续传代实验,确定了1株突变性状能稳定遗传的突变株,产量可达8.6g/L(干重),比原出发菌株产量提高了20%。The Acetobacter xylinum strain preserved by our laboratory, as the original strain, was mutated using UV mutagenesis in order to obtain a low temperature-resistant and high cellulose-producing mutant strain. One mutant strain, which was steady in hereditary, was obtained by preliminary screening and second screening, as well as serial passage. The yield of cellulose by the mutated strain reached 8.6 g/L in dry weight, which was 20% higher than that produced by the starting strain.

关 键 词：紫外诱变 细菌纤维素 低温 木醋杆菌 

分 类 号：Q933[生物学—微生物学]