分光光度法测定大曲糖化酶活力探讨  

Determination of the glucoamylase activity of Daqu by DNS

作　　者：马歌丽[1] 魏泉增[1] 张志刚 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]洛阳伊龙药业有限公司,河南洛阳417300

出　　处：《中国酿造》2008年第9期69-71,共3页China Brewing

摘　　要：采用分光光度计法测量了大曲的糖化酶活力。结果表明,糖化酶水解淀粉产生的葡萄糖与3,5-二硝基水杨酸(DNS)反应生成的棕红色3-氨基-5硝基-水杨酸,在波长520nm处的光吸收强度与糖化酶活力呈正比。The activity of glucoamylase in Daqu was determined by spectrophotometer, The results showed that the absorbance of 3-amino-5-nitro-salicylic acid, which was formed from the reaction of glucose produced by hydrolysis of starch granules by glucoamylase and 3,5-dinitrosalicylic acid, was proportion to the activity of glucoamylase at 520 nm. The method could quickly determine the activity of glucoamylase due to its simple process.

关 键 词：分光光度计 大曲 糖化酶活力 测定 

分 类 号：O657.3[理学—分析化学]